Secreted ferritin: Mosquito defense against iron overload?

Dawn L. Geiser, Dianzheng Zhang, Joy Winzerling

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The yellow fever mosquito, Aedes aegypti, must blood feed in order to complete her life cycle. The blood meal provides a high level of iron that is required for egg development. We are interested in developing control strategies that interfere with this process. We show that A. aegypti larval cells synthesize and secrete ferritin in response to iron exposure. Cytoplasmic ferritin is maximal at low levels of iron, consists of both the light chain (LCH) and heavy chain (HCH) subunits and reflects cytoplasmic iron levels. Secreted ferritin increases in direct linear relationship to iron dose and consists primarily of HCH subunits. Although the messages for both subunits increase with iron treatment, our data indicate that mosquito HCH synthesis could be partially controlled at the translational level as well. Importantly, we show that exposure of mosquito cells to iron at low concentrations increases cytoplasmic iron, while higher iron levels results in a decline in cytoplasmic iron levels indicating that excess iron is removed from mosquito cells. Our work indicates that HCH synthesis and ferritin secretion are key factors in the response of mosquito cells to iron exposure and could be the primary mechanisms that allow these insects to defend against an intracellular iron overload.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume36
Issue number3
DOIs
StatePublished - Mar 2006

Fingerprint

iron overload
Iron Overload
ferritin
Ferritins
Culicidae
Iron
iron
Aedes aegypti
Blood
cells
Apoferritins
Yellow Fever
synthesis
Aedes
blood meal
Life Cycle Stages
Ovum
Insects
Meals

Keywords

  • Aedes aegypti mosquito
  • Ferritin
  • Iron metabolism
  • Iron regulatory protein 1
  • Secretion

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Secreted ferritin : Mosquito defense against iron overload? / Geiser, Dawn L.; Zhang, Dianzheng; Winzerling, Joy.

In: Insect Biochemistry and Molecular Biology, Vol. 36, No. 3, 03.2006, p. 177-187.

Research output: Contribution to journalArticle

Geiser, Dawn L. ; Zhang, Dianzheng ; Winzerling, Joy. / Secreted ferritin : Mosquito defense against iron overload?. In: Insect Biochemistry and Molecular Biology. 2006 ; Vol. 36, No. 3. pp. 177-187.
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