Segregation distortion in Arabidopsis gametophytic factor 1 (gfa1) mutants is caused by a deficiency of an essential RNA splicing factor

Daniel A. Coury, Changqing Zhang, Ara Ko, Megan I. Skaggs, Cory A. Christensen, Gary N. Drews, Kenneth A Feldmann, Ramin Yadegari

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The female and male gametophytes are critical components of the angiosperm life cycle and are essential for the reproductive process. The gametophytes share many essential cellular processes with each other and with the sporophyte generation. As a consequence, these processes can only be analyzed genetically in the gametophyte generation. Here, we report the characterization of the gametophytic factor 1 (gfa1) mutant. The gfa1 mutation exhibits reduced transmission through both the female and male gametophytes. Reduced transmission through the female gametophyte is due to an effect on female gametophyte development. By contrast, development of the pollen grain is not affected in gfa1; rather, reduced transmission is likely due to an effect on pollen tube growth. We have identified multiple T-DNA-insertion alleles of gfa1 in a gene encoding a protein with high similarity to Snu114/U5-116 kD proteins from yeast and animals required for normal pre-mRNA splicing. Consistent with its predicted function, the GFA1 gene (At1g06220) is expressed throughout the plant. Together, these data suggest that GFA1 functions in mRNA splicing during the plant life cycle.

Original languageEnglish (US)
Pages (from-to)87-97
Number of pages11
JournalSexual Plant Reproduction
Volume20
Issue number2
DOIs
StatePublished - Jun 2007

Fingerprint

RNA splicing
segregation distortion
gametophytes
Pollen
Ovule
Arabidopsis
Life Cycle Stages
mutants
life cycle (organisms)
Plant Germ Cells
Pollen Tube
Angiosperms
Fungal Proteins
RNA Precursors
gametogenesis
pollen tubes
Genes
Angiospermae
genes
proteins

Keywords

  • Arabidopsis
  • Female gametophyte
  • Male gametophyte
  • RNA splicing
  • Segregation distortion

ASJC Scopus subject areas

  • Plant Science

Cite this

Segregation distortion in Arabidopsis gametophytic factor 1 (gfa1) mutants is caused by a deficiency of an essential RNA splicing factor. / Coury, Daniel A.; Zhang, Changqing; Ko, Ara; Skaggs, Megan I.; Christensen, Cory A.; Drews, Gary N.; Feldmann, Kenneth A; Yadegari, Ramin.

In: Sexual Plant Reproduction, Vol. 20, No. 2, 06.2007, p. 87-97.

Research output: Contribution to journalArticle

Coury, Daniel A. ; Zhang, Changqing ; Ko, Ara ; Skaggs, Megan I. ; Christensen, Cory A. ; Drews, Gary N. ; Feldmann, Kenneth A ; Yadegari, Ramin. / Segregation distortion in Arabidopsis gametophytic factor 1 (gfa1) mutants is caused by a deficiency of an essential RNA splicing factor. In: Sexual Plant Reproduction. 2007 ; Vol. 20, No. 2. pp. 87-97.
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