Selection and partial characterization of a Pseudomonas aeruginosa mono- rhamnolipid deficient mutant

Marina Wild, Alma Delia Caro, Ana Lilia Hernández, Raina M. Miller, Gloria Soberón-Chávez

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Pseudomonas aeruginosa produces rhamnolipids which are tenso-active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl-β- hydroxydecanoyl-β-hydroxydecanoate (mono-rhamnolipid) and rhamnosyl- rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (di-rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono-rhamnolipid while still producing di- rhamnolipid. IBT8 was selected after random mutagenesis with Tn501; yet, its mono-rhamnolipid deficiency was found associated neither with its Tn501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl- transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalFEMS Microbiology Letters
Volume153
Issue number2
DOIs
StatePublished - Aug 15 1997

Keywords

  • Pseudomonas aeruginosa
  • Rhamnolipid
  • rhl gene

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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