Selection of somatic hybrid plants in nicotiana through fluorescence-activated sorting of protoplasts

Claudio L. Afonso, Kristi R. Harkins, Mary A. Thomas-Compton, Ann E. Krejci, David W Galbraith

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We have developed general techniques for the successful selection of plant heterokaryons from parental populations of plant protoplasts subjected to somatic fusion treatments. These techniques involve a combination of artificial fluorescence labeling, natural chlorophyll autofluorescence and fluorescence-activated cell sorting. We have recovered purified heterokaryons at high rates and viabilities, and these were regenerated into plants. This quick and routine technique has allowed the recovery and unambiguous characterization of three different interspecific somatic hybrids of Nicotiana.

Original languageEnglish (US)
Pages (from-to)811-816
Number of pages6
JournalBio/Technology
Volume3
Issue number9
DOIs
StatePublished - 1985
Externally publishedYes

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Protoplasts
Sorting
Tobacco
Fluorescence
Chlorophyll
Labeling
Fusion reactions
Cells
Recovery
Flow Cytometry
Population

ASJC Scopus subject areas

  • Biotechnology

Cite this

Selection of somatic hybrid plants in nicotiana through fluorescence-activated sorting of protoplasts. / Afonso, Claudio L.; Harkins, Kristi R.; Thomas-Compton, Mary A.; Krejci, Ann E.; Galbraith, David W.

In: Bio/Technology, Vol. 3, No. 9, 1985, p. 811-816.

Research output: Contribution to journalArticle

Afonso, Claudio L. ; Harkins, Kristi R. ; Thomas-Compton, Mary A. ; Krejci, Ann E. ; Galbraith, David W. / Selection of somatic hybrid plants in nicotiana through fluorescence-activated sorting of protoplasts. In: Bio/Technology. 1985 ; Vol. 3, No. 9. pp. 811-816.
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