Selection on synonymous sites for increased accessibility around mirna binding sites in plants

Wanjun Gu, Xiaofei Wang, Chuanying Zhai, Xueying Xie, Tong Zhou

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Synonymous codons are widely selected for various biological mechanisms in both prokaryotes and eukaryotes. Recent evidence suggests that microRNA (miRNA) function may affect synonymous codon choices near miRNA target sites. To better understand this, we perform genome-wide analysis on synonymous codon usage around miRNA target sites in four plant genomes. We observed a general trend of increased site accessibility around miRNA target sites in plants. Guanine-cytosine (GC)-poor codons are preferred in the flank region of miRNA target sites. Within-genome analyses show significant variation among miRNA targets in species. GC content of the target gene can partly explain the variation of site accessibility among miRNA targets. miRNA targets in GC-rich genes show stronger selection signals than those in GC-poor genes. Gene's codon usage bias and the conservation level of miRNA and its target also have some effects on site accessibility, but the expression level of miRNA or its target and the mechanism of miRNA activity do not contribute to site accessibility differences among miRNA targets. We suggest that synonymous codons near miRNA targets are selected for efficient miRNA binding and proper miRNA function. Our results present a new dimension of natural selection on synonymous codons near miRNA target sites in plants, which will have important implications of coding sequence evolution.

Original languageEnglish (US)
Pages (from-to)3037-3044
Number of pages8
JournalMolecular Biology and Evolution
Volume29
Issue number10
DOIs
StatePublished - Oct 2012
Externally publishedYes

Fingerprint

MicroRNAs
microRNA
accessibility
binding sites
Binding Sites
genome
gene
codons
Codon
cytosine
guanine
prokaryote
eukaryote
natural selection
Cytosine
Guanine
Genes
genes
Genome
Plant Genome

Keywords

  • microRNA function
  • site accessibility
  • synonymous selection

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Selection on synonymous sites for increased accessibility around mirna binding sites in plants. / Gu, Wanjun; Wang, Xiaofei; Zhai, Chuanying; Xie, Xueying; Zhou, Tong.

In: Molecular Biology and Evolution, Vol. 29, No. 10, 10.2012, p. 3037-3044.

Research output: Contribution to journalArticle

Gu, Wanjun ; Wang, Xiaofei ; Zhai, Chuanying ; Xie, Xueying ; Zhou, Tong. / Selection on synonymous sites for increased accessibility around mirna binding sites in plants. In: Molecular Biology and Evolution. 2012 ; Vol. 29, No. 10. pp. 3037-3044.
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