Selective 4′-O-methylglycosylation of the pentahydroxy-flavonoid quercetin by Beauveria bassiana ATCC 7159

Jixun Zhan, Leslie Gunatilaka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Biotransformation of the pentahydroxy-flavonoid natural product, quercetin, by Beauveria bassiana ATCC 7159 afforded a new derivative, quercetin-4′-O-methyl-7-β-D-glucopyranoside, in 87% isolated yield suggesting that glucosylation of the substrate occurred with high selectivity at C-7-OH out of the five hydroxyl groups. Most of the product was isolated from the mycelium and the filtrate of the culture medium did not show any catalytic activity. The mycelium is capable of performing this biotransformation when suspended in buffers of pH 2.1 and 7.2, suggesting that intracellular enzymes are involved and that they are active at a wide range of extracellular pH.

Original languageEnglish (US)
Pages (from-to)396-399
Number of pages4
JournalBiocatalysis and Biotransformation
Volume24
Issue number5
DOIs
StatePublished - Sep 2006

Fingerprint

Beauveria
Flavonoids
Mycelium
Quercetin
Biotransformation
Biological Products
Hydroxyl Radical
Culture Media
Catalyst activity
Buffers
Enzymes
Derivatives
Substrates
methylglucoside

Keywords

  • 4′-O-methyl-glycosylation
  • Intracellular enzymes
  • Quercetin
  • Selectivity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Catalysis

Cite this

Selective 4′-O-methylglycosylation of the pentahydroxy-flavonoid quercetin by Beauveria bassiana ATCC 7159. / Zhan, Jixun; Gunatilaka, Leslie.

In: Biocatalysis and Biotransformation, Vol. 24, No. 5, 09.2006, p. 396-399.

Research output: Contribution to journalArticle

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