Selective decline in protein F1 phosphorylation in hippocampus of senescent rats

Carol A Barnes, S. J Y Mizumori, D. M. Lovinger, F. S. Sheu, K. Murakami, S. Y. Chan, D. J. Linden, R. B. Nelson, A. Routtenberg

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Certain forms of neuronal plasticity have been found to be expressed through alterations in brain protein phosphorylation, and its regulation by protein kinase activity. Of interest in this regard is the possibility that the decline in neuronal plasticity and cognitive function that occurs in advanced age may result in part from altered phosphorylation of specific proteins. As a first attempt to identify age-related changes in phosphoproteins, we assayed in vitro phosphorylation of proteins in hippocampus, cerebellum, entorhinal cortex, and frontal cortex from Fischer-344 rats of 5 months, I I months, and 25 months of age. Compared to the middle-aged animals, the aged rats showed a selective 46% decline in phosphorylation of the 47 kDa protein (F1) in hippocampus, with no change in the phosphorylation of other proteins measured in this structure. Aged animals also showed decreased phosphorylation relative to young animals. No age-related change was observed in any protein band for the other brain areas examined. Since protein F1 is phosphorylated by protein kinase C (PKC), the cytosolic and membrane distribution of this enzyme was compared across age groups. The activity of PKC in hippocampus did not change across age. The explanation of this age-related decline in protein F1 phosphorylation is likely to be a decline in the substrate protein itself. The results are discussed in terms of protein F1's possible role in age-related decline of hippocampal synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalNeurobiology of Aging
Volume9
Issue numberC
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Hippocampus
Phosphorylation
Proteins
Neuronal Plasticity
Protein Kinase C
Entorhinal Cortex
Phosphoproteins
Inbred F344 Rats
Brain
Frontal Lobe
Cerebellum
Protein Kinases
Cognition
Age Groups
Membranes
Enzymes

Keywords

  • Aging
  • Hippocampus
  • Protein F1
  • Protein kinase C
  • Protein phosphorylation

ASJC Scopus subject areas

  • Aging
  • Developmental Biology
  • Geriatrics and Gerontology
  • Clinical Neurology
  • Neuroscience(all)
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Barnes, C. A., Mizumori, S. J. Y., Lovinger, D. M., Sheu, F. S., Murakami, K., Chan, S. Y., ... Routtenberg, A. (1988). Selective decline in protein F1 phosphorylation in hippocampus of senescent rats. Neurobiology of Aging, 9(C), 393-398. https://doi.org/10.1016/S0197-4580(88)80086-1

Selective decline in protein F1 phosphorylation in hippocampus of senescent rats. / Barnes, Carol A; Mizumori, S. J Y; Lovinger, D. M.; Sheu, F. S.; Murakami, K.; Chan, S. Y.; Linden, D. J.; Nelson, R. B.; Routtenberg, A.

In: Neurobiology of Aging, Vol. 9, No. C, 1988, p. 393-398.

Research output: Contribution to journalArticle

Barnes, CA, Mizumori, SJY, Lovinger, DM, Sheu, FS, Murakami, K, Chan, SY, Linden, DJ, Nelson, RB & Routtenberg, A 1988, 'Selective decline in protein F1 phosphorylation in hippocampus of senescent rats', Neurobiology of Aging, vol. 9, no. C, pp. 393-398. https://doi.org/10.1016/S0197-4580(88)80086-1
Barnes, Carol A ; Mizumori, S. J Y ; Lovinger, D. M. ; Sheu, F. S. ; Murakami, K. ; Chan, S. Y. ; Linden, D. J. ; Nelson, R. B. ; Routtenberg, A. / Selective decline in protein F1 phosphorylation in hippocampus of senescent rats. In: Neurobiology of Aging. 1988 ; Vol. 9, No. C. pp. 393-398.
@article{29356a1523c8407a97626d85b6ed9aba,
title = "Selective decline in protein F1 phosphorylation in hippocampus of senescent rats",
abstract = "Certain forms of neuronal plasticity have been found to be expressed through alterations in brain protein phosphorylation, and its regulation by protein kinase activity. Of interest in this regard is the possibility that the decline in neuronal plasticity and cognitive function that occurs in advanced age may result in part from altered phosphorylation of specific proteins. As a first attempt to identify age-related changes in phosphoproteins, we assayed in vitro phosphorylation of proteins in hippocampus, cerebellum, entorhinal cortex, and frontal cortex from Fischer-344 rats of 5 months, I I months, and 25 months of age. Compared to the middle-aged animals, the aged rats showed a selective 46{\%} decline in phosphorylation of the 47 kDa protein (F1) in hippocampus, with no change in the phosphorylation of other proteins measured in this structure. Aged animals also showed decreased phosphorylation relative to young animals. No age-related change was observed in any protein band for the other brain areas examined. Since protein F1 is phosphorylated by protein kinase C (PKC), the cytosolic and membrane distribution of this enzyme was compared across age groups. The activity of PKC in hippocampus did not change across age. The explanation of this age-related decline in protein F1 phosphorylation is likely to be a decline in the substrate protein itself. The results are discussed in terms of protein F1's possible role in age-related decline of hippocampal synaptic plasticity.",
keywords = "Aging, Hippocampus, Protein F1, Protein kinase C, Protein phosphorylation",
author = "Barnes, {Carol A} and Mizumori, {S. J Y} and Lovinger, {D. M.} and Sheu, {F. S.} and K. Murakami and Chan, {S. Y.} and Linden, {D. J.} and Nelson, {R. B.} and A. Routtenberg",
year = "1988",
doi = "10.1016/S0197-4580(88)80086-1",
language = "English (US)",
volume = "9",
pages = "393--398",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",
number = "C",

}

TY - JOUR

T1 - Selective decline in protein F1 phosphorylation in hippocampus of senescent rats

AU - Barnes, Carol A

AU - Mizumori, S. J Y

AU - Lovinger, D. M.

AU - Sheu, F. S.

AU - Murakami, K.

AU - Chan, S. Y.

AU - Linden, D. J.

AU - Nelson, R. B.

AU - Routtenberg, A.

PY - 1988

Y1 - 1988

N2 - Certain forms of neuronal plasticity have been found to be expressed through alterations in brain protein phosphorylation, and its regulation by protein kinase activity. Of interest in this regard is the possibility that the decline in neuronal plasticity and cognitive function that occurs in advanced age may result in part from altered phosphorylation of specific proteins. As a first attempt to identify age-related changes in phosphoproteins, we assayed in vitro phosphorylation of proteins in hippocampus, cerebellum, entorhinal cortex, and frontal cortex from Fischer-344 rats of 5 months, I I months, and 25 months of age. Compared to the middle-aged animals, the aged rats showed a selective 46% decline in phosphorylation of the 47 kDa protein (F1) in hippocampus, with no change in the phosphorylation of other proteins measured in this structure. Aged animals also showed decreased phosphorylation relative to young animals. No age-related change was observed in any protein band for the other brain areas examined. Since protein F1 is phosphorylated by protein kinase C (PKC), the cytosolic and membrane distribution of this enzyme was compared across age groups. The activity of PKC in hippocampus did not change across age. The explanation of this age-related decline in protein F1 phosphorylation is likely to be a decline in the substrate protein itself. The results are discussed in terms of protein F1's possible role in age-related decline of hippocampal synaptic plasticity.

AB - Certain forms of neuronal plasticity have been found to be expressed through alterations in brain protein phosphorylation, and its regulation by protein kinase activity. Of interest in this regard is the possibility that the decline in neuronal plasticity and cognitive function that occurs in advanced age may result in part from altered phosphorylation of specific proteins. As a first attempt to identify age-related changes in phosphoproteins, we assayed in vitro phosphorylation of proteins in hippocampus, cerebellum, entorhinal cortex, and frontal cortex from Fischer-344 rats of 5 months, I I months, and 25 months of age. Compared to the middle-aged animals, the aged rats showed a selective 46% decline in phosphorylation of the 47 kDa protein (F1) in hippocampus, with no change in the phosphorylation of other proteins measured in this structure. Aged animals also showed decreased phosphorylation relative to young animals. No age-related change was observed in any protein band for the other brain areas examined. Since protein F1 is phosphorylated by protein kinase C (PKC), the cytosolic and membrane distribution of this enzyme was compared across age groups. The activity of PKC in hippocampus did not change across age. The explanation of this age-related decline in protein F1 phosphorylation is likely to be a decline in the substrate protein itself. The results are discussed in terms of protein F1's possible role in age-related decline of hippocampal synaptic plasticity.

KW - Aging

KW - Hippocampus

KW - Protein F1

KW - Protein kinase C

KW - Protein phosphorylation

UR - http://www.scopus.com/inward/record.url?scp=0024161070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024161070&partnerID=8YFLogxK

U2 - 10.1016/S0197-4580(88)80086-1

DO - 10.1016/S0197-4580(88)80086-1

M3 - Article

VL - 9

SP - 393

EP - 398

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

IS - C

ER -