Selective enrichment of Geobacter sulfurreducens from anaerobic granular sludge with quinones as terminal electron acceptors

Francisco J. Cervantes, Tuan Duong-Dac, Anna E. Ivanova, Kees Roest, Antoon D.L. Akkermans, Gatze Lettinga, Jim A. Field

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A quinone-respiring, enrichment culture derived from methanogenic granular sludge was phylogenetically characterized by using a combined cloning-denaturing gradient gel electrophoresis (DGGE) method, which revealed that the consortium developed was dominated by a single microorganism: 97% related, in a sequence of 1520 base pairs, to Geobacter sulfurreducens. The enrichment culture could grow with acetate, formate or H2 when humic acids, the humic model compound, anthraquinone-2,6-disulfonate (AQDS), or chelated Fe(III) was provided as a terminal electron acceptor. The occurrence of a humic acid- or quinone-respiring microorganism in the microbial community of a wastewater treatment system suggests that this type of microorganisms may play a potential role in anaerobic bioreactors treating humus-containing wastewaters.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalBiotechnology Letters
Volume25
Issue number1
DOIs
StatePublished - Jan 1 2003

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Keywords

  • 6-disulfonate
  • Anthraquinone-2
  • Geobacter sulfurreducens
  • Granular sludge
  • Humus reduction
  • Wastewater

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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