Abstract
An enhanced version of the X(ω1) half-filtered TOCSY experiment for measurement of long-range heteronuclear coupling constants is proposed which yields high-quality spectra with substantially increased sensitivity and resolution. The modified method features gradient-enhanced X filtering sequences, broadband homonuclear decoupling during t1, optional 1JXII scaling in the F1 domain, and gradient coherence selection in combination with the sensitivity-enhanced protocol for the TOCSY transfer. These modifications extend the applicability of the method - coupling constants can be measured accurately for natural abundance samples at low concentrations and for compounds yielding complex spectra. Computer-aided analysis of E.COSY-type multiplets is applied for the determination of heteronuclear long-range coupling constants.
Original language | English (US) |
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Pages (from-to) | 155-161 |
Number of pages | 7 |
Journal | Journal of Magnetic Resonance |
Volume | 130 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1998 |
Keywords
- Echo-antiecho selection
- Gradient-enhanced X filtering
- Long-range heteronuclear couplings
- Sensitivity enhancement
- X(ω) half-filtered TOCSY
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Nuclear and High Energy Physics
- Condensed Matter Physics