Sensitivity- and Gradient-Enhanced Hetero (ω1) Half-Filtered TOCSY Experiment for Measuring Long-Range Heteronuclear Coupling Constants

Dušan Uhrín, Gyula Batta, Victor J. Hruby, Paul N. Barlow, Katalin E. Kövér

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

An enhanced version of the X(ω1) half-filtered TOCSY experiment for measurement of long-range heteronuclear coupling constants is proposed which yields high-quality spectra with substantially increased sensitivity and resolution. The modified method features gradient-enhanced X filtering sequences, broadband homonuclear decoupling during t1, optional 1JXII scaling in the F1 domain, and gradient coherence selection in combination with the sensitivity-enhanced protocol for the TOCSY transfer. These modifications extend the applicability of the method - coupling constants can be measured accurately for natural abundance samples at low concentrations and for compounds yielding complex spectra. Computer-aided analysis of E.COSY-type multiplets is applied for the determination of heteronuclear long-range coupling constants.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalJournal of Magnetic Resonance
Volume130
Issue number2
DOIs
StatePublished - Feb 1998

    Fingerprint

Keywords

  • Echo-antiecho selection
  • Gradient-enhanced X filtering
  • Long-range heteronuclear couplings
  • Sensitivity enhancement
  • X(ω) half-filtered TOCSY

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Cite this