Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts

Kathryn M. Miller, Charles R Sterling

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

Original languageEnglish (US)
Pages (from-to)5949-5950
Number of pages2
JournalApplied and Environmental Microbiology
Volume73
Issue number18
DOIs
StatePublished - Sep 2007

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Giardia lamblia
cyst
Cysts
Polymerase Chain Reaction
amplification
detection

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts. / Miller, Kathryn M.; Sterling, Charles R.

In: Applied and Environmental Microbiology, Vol. 73, No. 18, 09.2007, p. 5949-5950.

Research output: Contribution to journalArticle

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