Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation

S. Kim Ratanathanawongs Williams, Gregory M. Raner, Walther R. Ellis, J. Calvin Giddings

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalJournal of Microcolumn Separations
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • Cell lysates
  • Field-flow fractionation
  • Inclusion bodies
  • Recombinant proteins
  • Refractile bodies

ASJC Scopus subject areas

  • Mechanical Engineering
  • Filtration and Separation

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