Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation

S. Kim Ratanathanawongs Williams, Gregory M. Raner, Walther R Ellis, J. Calvin Giddings

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalJournal of Microcolumn Separations
Volume9
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Fractionation
Sedimentation
Escherichia coli
Flow fields
Proteins
Sodium dodecyl sulfate
Polyacrylates
Electrophoresis
Sodium Dodecyl Sulfate
Gels
myohemerythrin
polyacrylamide gels

Keywords

  • Cell lysates
  • Field-flow fractionation
  • Inclusion bodies
  • Recombinant proteins
  • Refractile bodies

ASJC Scopus subject areas

  • Filtration and Separation
  • Mechanical Engineering

Cite this

Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation. / Ratanathanawongs Williams, S. Kim; Raner, Gregory M.; Ellis, Walther R; Giddings, J. Calvin.

In: Journal of Microcolumn Separations, Vol. 9, No. 3, 1997, p. 233-239.

Research output: Contribution to journalArticle

Ratanathanawongs Williams, S. Kim ; Raner, Gregory M. ; Ellis, Walther R ; Giddings, J. Calvin. / Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation. In: Journal of Microcolumn Separations. 1997 ; Vol. 9, No. 3. pp. 233-239.
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