Separation of serum proteins with uncoupled microsphere particles in a stirred cell

Jeong-Yeol Yoon, Jung Hun Lee, Jung Hyun Kim, Woo Sik Kim

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Submicron microspheres were used directly without ligand coupling for the batch and continuous separations of proteins. In the batch experiments for separating BSA (bovine serum albumin) from BHb (bovine hemoglobin), introducing both hydrophobic effects for BSA and electrostatic repulsion for BHb (and vice versa) was required for high selectivity, and microspheres with low number density of surface groups were advantageous. For the continuous experiments, the utilization of a stirred cell was successful, where the microspheres were in the form of latex with good dispersion of particles. The flow rate without a pump was 0.5-1.3 ml min-1, and the ratio of BSA and BHb was varied. In the experiments for eliminating BHb from BSA, elution curves of BHb corresponded to the single component breakthrough curves, while those for BSA did not. The latter is believed to be due to the interference by BHb in the adsorption of BSA.

Original languageEnglish (US)
Pages (from-to)365-377
Number of pages13
JournalColloids and Surfaces B: Biointerfaces
Volume10
Issue number6
DOIs
StatePublished - May 15 1998
Externally publishedYes

Fingerprint

Hemoglobin
hemoglobin
Bovine Serum Albumin
Microspheres
albumins
serums
Blood Proteins
Hemoglobins
proteins
Proteins
cells
elution
Experiments
Latex
curves
latex
Static Electricity
Latexes
Particles (particulate matter)
Adsorption

Keywords

  • Bovine hemoglobin
  • Bovine serum albumin
  • Microspheres
  • Protein separation
  • Stirred cell

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Separation of serum proteins with uncoupled microsphere particles in a stirred cell. / Yoon, Jeong-Yeol; Lee, Jung Hun; Kim, Jung Hyun; Kim, Woo Sik.

In: Colloids and Surfaces B: Biointerfaces, Vol. 10, No. 6, 15.05.1998, p. 365-377.

Research output: Contribution to journalArticle

Yoon, Jeong-Yeol ; Lee, Jung Hun ; Kim, Jung Hyun ; Kim, Woo Sik. / Separation of serum proteins with uncoupled microsphere particles in a stirred cell. In: Colloids and Surfaces B: Biointerfaces. 1998 ; Vol. 10, No. 6. pp. 365-377.
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