Separation of serum proteins with uncoupled microsphere particles in a stirred cell

Jeong Yeol Yoon, Jung Hun Lee, Jung Hyun Kim, Woo Sik Kim

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Submicron microspheres were used directly without ligand coupling for the batch and continuous separations of proteins. In the batch experiments for separating BSA (bovine serum albumin) from BHb (bovine hemoglobin), introducing both hydrophobic effects for BSA and electrostatic repulsion for BHb (and vice versa) was required for high selectivity, and microspheres with low number density of surface groups were advantageous. For the continuous experiments, the utilization of a stirred cell was successful, where the microspheres were in the form of latex with good dispersion of particles. The flow rate without a pump was 0.5-1.3 ml min-1, and the ratio of BSA and BHb was varied. In the experiments for eliminating BHb from BSA, elution curves of BHb corresponded to the single component breakthrough curves, while those for BSA did not. The latter is believed to be due to the interference by BHb in the adsorption of BSA.

Original languageEnglish (US)
Pages (from-to)365-377
Number of pages13
JournalColloids and Surfaces B: Biointerfaces
Volume10
Issue number6
DOIs
StatePublished - May 15 1998

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Keywords

  • Bovine hemoglobin
  • Bovine serum albumin
  • Microspheres
  • Protein separation
  • Stirred cell

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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