Sequence composition and genome organization of maize

Joachim Messing, Arvind K. Bharti, Wojciech M. Karlowski, Heidrun Gundlach, Ran Kim Hye, Yeisoo Yu, Fusheng Wei, Galina Fuks, Carol A. Soderlund, Klaus F X Mayer, Rod A Wing

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

Zea mays L. ssp. mays, or corn, one of the most important crops and a model for plant genetics, has a genome ≈80% the size of the human genome. To gain global insight into the organization of its genome, we have sequenced the ends of large insert clones, yielding a cumulative length of one-eighth of the genome with a DNA sequence read every 6.2 kb, thereby describing a large percentage of the genes and transposable elements of maize in an unbiased approach. Based on the accumulative 307 Mb of sequence, repeat sequences occupy 58% and genic regions occupy 7.5%. A conservative estimate predicts ≈59,000 genes, which is higher than in any other organism sequenced so far. Because the sequences are derived from bacterial artificial chromosome clones, which are ordered in overlapping bins, tagged genes are also ordered along continuous chromosomal segments. Based on this positional information, roughly one-third of the genes appear to consist of tandemly arrayed gene families. Although the ancestor of maize arose by tetraploidization, fewer than half of the genes appear to be present in two orthologous copies, indicating that the maize genome has undergone significant gene loss since the duplication event.

Original languageEnglish (US)
Pages (from-to)14349-14354
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number40
DOIs
StatePublished - Oct 5 2004

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Zea mays
Genome
Genes
Clone Cells
Bacterial Artificial Chromosomes
Genome Size
DNA Transposable Elements
Genetic Models
Human Genome

Keywords

  • Gene families
  • Maize genome
  • Map-based sequence
  • Whole-genome duplication
  • Whole-genome sequence tags

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Sequence composition and genome organization of maize. / Messing, Joachim; Bharti, Arvind K.; Karlowski, Wojciech M.; Gundlach, Heidrun; Hye, Ran Kim; Yu, Yeisoo; Wei, Fusheng; Fuks, Galina; Soderlund, Carol A.; Mayer, Klaus F X; Wing, Rod A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 40, 05.10.2004, p. 14349-14354.

Research output: Contribution to journalArticle

Messing, J, Bharti, AK, Karlowski, WM, Gundlach, H, Hye, RK, Yu, Y, Wei, F, Fuks, G, Soderlund, CA, Mayer, KFX & Wing, RA 2004, 'Sequence composition and genome organization of maize', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 40, pp. 14349-14354. https://doi.org/10.1073/pnas.0406163101
Messing, Joachim ; Bharti, Arvind K. ; Karlowski, Wojciech M. ; Gundlach, Heidrun ; Hye, Ran Kim ; Yu, Yeisoo ; Wei, Fusheng ; Fuks, Galina ; Soderlund, Carol A. ; Mayer, Klaus F X ; Wing, Rod A. / Sequence composition and genome organization of maize. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 40. pp. 14349-14354.
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