Sequence determinants of a conformational switch in a protein structure

Thomas A. Anderson, Matthew Hj Cordes, Robert T. Sauer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The Arc repressor of bacteriophage P22 is a dimeric member of the ribbon-helix-helix family of transcription factors. Residues 9-14 of each wild-type Arc subunit pair to form two antiparallel -strands and have the alternating pattern of polar and nonpolar residues expected for a β-ribbon with one solvent-exposed face and one face that forms part of the hydrophobic core. Simultaneously switching Asn-11 to Leu and Leu-12 to Asn changes the local binary sequence pattern to that of an amphipathic helix. Previous studies have shown that this double mutation results in replacement of the wild-type β-ribbon by two right-handed Biohelices. Moreover, an Arc variant bearing just the Asn-11 → Leu mutation has an ambiguous binary pattern and can form either the ribbon or the helical structures, which interchange rapidly. Here, we study Arc mutants in which position 11 is occupied by Gly, Ala, Val, Ile, Leu, Met, Phe, or Tyr. These mutants adopt the wild-type β-ribbon structure in a sequence context that stabilizes this fold, but they assume the alternative helical structure in a sequence background in which the wild-type fold is precluded by negative design. In an otherwise wild-type sequence background, the detailed chemical properties of the position 11 side chain dictate which of the two competing conformational folds is preferred.

Original languageEnglish (US)
Pages (from-to)18344-18349
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number51
DOIs
StatePublished - Dec 20 2005

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Bacteriophage P22
Mutation
Proteins
Transcription Factors

Keywords

  • Protein folding
  • Secondary structure
  • Structural evolution

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Sequence determinants of a conformational switch in a protein structure. / Anderson, Thomas A.; Cordes, Matthew Hj; Sauer, Robert T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 51, 20.12.2005, p. 18344-18349.

Research output: Contribution to journalArticle

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