Sequential detergent extraction prior to mass spectrometry analysis.

Fiona M McCarthy, Amanda M. Cooksey, Shane C Burgess

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Sequential detergent extraction of proteins from eukaryotic cells has been used to increase proteome coverage of 2D-PAGE. We have adapted sequential detergent extraction for use with the high-throughput non-electrophoretic proteomics method of liquid chromatography and electrospray ionisation tandem mass spectrometry. This method of extraction yields comprehensive proteomes that include up to twice as many membrane proteins as other published methods. Two thirds of these membrane proteins have more than one transmembrane domain and many of these have multiple transmembrane domains. Since sequential detergent extraction (SDE) separates proteins based upon their physicochemistry and sub-cellular localisation, this method also provides useful data about cellular localisation.

Original languageEnglish (US)
Pages (from-to)110-118
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume528
DOIs
Publication statusPublished - 2009
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this