Sequestrocytes: A manifestation of transcellular cross-bonding of the red cell membrane in sickle cell anemia

Ronald S Weinstein, J. A. Warth, K. Near, Y. Marikovsky

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An unusual form of red cells (called sequestrocytes) that circulate in the peripheral blood of patients with sickle anemia has been identified. In wet-mount light microscopy preparations, sequestrocytes appear as massively vacuolated erythrocytes. We have shown by transmission electron microscopy and scanning electron microscopy that the morphology of sequestrocytes can be accounted for on the basis of transcellular cross-bonding of the cell membrane. Initially, endocytotic vesicles span the cytoplasmic space, forming putative cytoskeletal fusion zones. Points of fusion of the membrane skeleton bridging the cytoplasmic compartment expand laterally to form linear fusion zones that entrap lakes of hemoglobin within pseudovacuoles. Sequestrocytes can be isolated in the densest layer (1.159 g ml-1 or greater) of an arabinogalactan density gradient. These cells can be generated in increased numbers in sickle cell blood by incubating samples in 1.5mM-acetylphenylhydrazine (APH) solution for two hours at 37°C. Their information is partially blocked by incubation with the reducing agent, dithiothreitol (DTT). Our results suggest that these cells represent an expression of oxidative membrane injury in sickle cell anemia.

Original languageEnglish (US)
Pages (from-to)593-600
Number of pages8
JournalJournal of Cell Science
Volume94
Issue number3
StatePublished - 1989
Externally publishedYes

Fingerprint

Red Cross
Sickle Cell Anemia
Cell Membrane
Cytoplasmic Vesicles
Membrane Fusion
Dithiothreitol
Reducing Agents
Lakes
Transmission Electron Microscopy
Skeleton
Electron Scanning Microscopy
Anemia
Microscopy
Blood Cells
Hemoglobins
Erythrocytes
Light
Membranes
Wounds and Injuries

Keywords

  • Membrane cross-bonding
  • Oxidant injury
  • Sickle cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sequestrocytes : A manifestation of transcellular cross-bonding of the red cell membrane in sickle cell anemia. / Weinstein, Ronald S; Warth, J. A.; Near, K.; Marikovsky, Y.

In: Journal of Cell Science, Vol. 94, No. 3, 1989, p. 593-600.

Research output: Contribution to journalArticle

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