Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography

Lida P. Hariri, Ziping Qiu, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Natalia Ignatenko, Boris Považay, Boris Hermann, Harald Sattmann, James McNally, Angelika Unterhuber, Wolfgang Drexler, Jennifer K Barton

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be implemented in a small diameter endoscope for imaging mouse models of colorectal cancer (CRC). In this study, we utilized ultrahigh resolution (UHR) OCT to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC in order to monitor the progression of neoplastic transformations and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22 and 26 weeks post AOM treatment) using a 2.0 mm diameter UHR OCT endoscopic system with 3.2 mm axial and 4.4 mm lateral resolution. Histological samples obtained at the final timepoint served as the diagnostic reference. A blinded expert panel of mouse colon pathologists provided diagnoses from the OCT images based on criteria developed from a separate training set of OCT images. Panel results were compared to histological diagnoses assigned by a blinded pathologist. Results: At the final imaging timepoint, 95% of adenomas and 23% of gastrointestinal neoplasias (38% protruding GINs and 9% non-protruding GINs) were correctly diagnosed. The panel identified 68% of disease foci (95% adenoma, 76% protruding GINs and 13% non-protruding GINs). Over the OCT imaging timepoints, disease progression followed a typical succession, with normal or GIN preceding adenoma. Conclusions: Endoscopic UHR OCT enabled accurate diagnosis of adenomas, identification of protruding GIN and non-destructive visualization of CRC progression, providing a tool for cancer research in animal models.

Original languageEnglish (US)
Pages (from-to)1753-1762
Number of pages10
JournalCancer Biology and Therapy
Volume6
Issue number11
StatePublished - Nov 2007

Fingerprint

Azoxymethane
Optical Coherence Tomography
Endoscopy
Adenoma
Colorectal Neoplasms
Colon
Endoscopes
Disease Progression
Neoplasms
Research Design
Animal Models

Keywords

  • Animal models for carcinogenesis
  • Azoxymethane treated mouse model of colorectal neoplasm
  • Colorectal adenoma
  • Endoscopic imaging
  • Gastrointestinal cancer
  • Gastrointestinal intraepithelial neoplasia
  • Imaging of tumor progression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. / Hariri, Lida P.; Qiu, Ziping; Tumlinson, Alexandre R.; Besselsen, David G.; Gerner, Eugene W.; Ignatenko, Natalia; Považay, Boris; Hermann, Boris; Sattmann, Harald; McNally, James; Unterhuber, Angelika; Drexler, Wolfgang; Barton, Jennifer K.

In: Cancer Biology and Therapy, Vol. 6, No. 11, 11.2007, p. 1753-1762.

Research output: Contribution to journalArticle

Hariri, LP, Qiu, Z, Tumlinson, AR, Besselsen, DG, Gerner, EW, Ignatenko, N, Považay, B, Hermann, B, Sattmann, H, McNally, J, Unterhuber, A, Drexler, W & Barton, JK 2007, 'Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography', Cancer Biology and Therapy, vol. 6, no. 11, pp. 1753-1762.
Hariri, Lida P. ; Qiu, Ziping ; Tumlinson, Alexandre R. ; Besselsen, David G. ; Gerner, Eugene W. ; Ignatenko, Natalia ; Považay, Boris ; Hermann, Boris ; Sattmann, Harald ; McNally, James ; Unterhuber, Angelika ; Drexler, Wolfgang ; Barton, Jennifer K. / Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. In: Cancer Biology and Therapy. 2007 ; Vol. 6, No. 11. pp. 1753-1762.
@article{0134e342a8a442348cd4cff063c1b2c0,
title = "Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography",
abstract = "Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be implemented in a small diameter endoscope for imaging mouse models of colorectal cancer (CRC). In this study, we utilized ultrahigh resolution (UHR) OCT to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC in order to monitor the progression of neoplastic transformations and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22 and 26 weeks post AOM treatment) using a 2.0 mm diameter UHR OCT endoscopic system with 3.2 mm axial and 4.4 mm lateral resolution. Histological samples obtained at the final timepoint served as the diagnostic reference. A blinded expert panel of mouse colon pathologists provided diagnoses from the OCT images based on criteria developed from a separate training set of OCT images. Panel results were compared to histological diagnoses assigned by a blinded pathologist. Results: At the final imaging timepoint, 95{\%} of adenomas and 23{\%} of gastrointestinal neoplasias (38{\%} protruding GINs and 9{\%} non-protruding GINs) were correctly diagnosed. The panel identified 68{\%} of disease foci (95{\%} adenoma, 76{\%} protruding GINs and 13{\%} non-protruding GINs). Over the OCT imaging timepoints, disease progression followed a typical succession, with normal or GIN preceding adenoma. Conclusions: Endoscopic UHR OCT enabled accurate diagnosis of adenomas, identification of protruding GIN and non-destructive visualization of CRC progression, providing a tool for cancer research in animal models.",
keywords = "Animal models for carcinogenesis, Azoxymethane treated mouse model of colorectal neoplasm, Colorectal adenoma, Endoscopic imaging, Gastrointestinal cancer, Gastrointestinal intraepithelial neoplasia, Imaging of tumor progression",
author = "Hariri, {Lida P.} and Ziping Qiu and Tumlinson, {Alexandre R.} and Besselsen, {David G.} and Gerner, {Eugene W.} and Natalia Ignatenko and Boris Považay and Boris Hermann and Harald Sattmann and James McNally and Angelika Unterhuber and Wolfgang Drexler and Barton, {Jennifer K}",
year = "2007",
month = "11",
language = "English (US)",
volume = "6",
pages = "1753--1762",
journal = "Cancer Biology and Therapy",
issn = "1538-4047",
publisher = "Landes Bioscience",
number = "11",

}

TY - JOUR

T1 - Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography

AU - Hariri, Lida P.

AU - Qiu, Ziping

AU - Tumlinson, Alexandre R.

AU - Besselsen, David G.

AU - Gerner, Eugene W.

AU - Ignatenko, Natalia

AU - Považay, Boris

AU - Hermann, Boris

AU - Sattmann, Harald

AU - McNally, James

AU - Unterhuber, Angelika

AU - Drexler, Wolfgang

AU - Barton, Jennifer K

PY - 2007/11

Y1 - 2007/11

N2 - Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be implemented in a small diameter endoscope for imaging mouse models of colorectal cancer (CRC). In this study, we utilized ultrahigh resolution (UHR) OCT to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC in order to monitor the progression of neoplastic transformations and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22 and 26 weeks post AOM treatment) using a 2.0 mm diameter UHR OCT endoscopic system with 3.2 mm axial and 4.4 mm lateral resolution. Histological samples obtained at the final timepoint served as the diagnostic reference. A blinded expert panel of mouse colon pathologists provided diagnoses from the OCT images based on criteria developed from a separate training set of OCT images. Panel results were compared to histological diagnoses assigned by a blinded pathologist. Results: At the final imaging timepoint, 95% of adenomas and 23% of gastrointestinal neoplasias (38% protruding GINs and 9% non-protruding GINs) were correctly diagnosed. The panel identified 68% of disease foci (95% adenoma, 76% protruding GINs and 13% non-protruding GINs). Over the OCT imaging timepoints, disease progression followed a typical succession, with normal or GIN preceding adenoma. Conclusions: Endoscopic UHR OCT enabled accurate diagnosis of adenomas, identification of protruding GIN and non-destructive visualization of CRC progression, providing a tool for cancer research in animal models.

AB - Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be implemented in a small diameter endoscope for imaging mouse models of colorectal cancer (CRC). In this study, we utilized ultrahigh resolution (UHR) OCT to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC in order to monitor the progression of neoplastic transformations and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22 and 26 weeks post AOM treatment) using a 2.0 mm diameter UHR OCT endoscopic system with 3.2 mm axial and 4.4 mm lateral resolution. Histological samples obtained at the final timepoint served as the diagnostic reference. A blinded expert panel of mouse colon pathologists provided diagnoses from the OCT images based on criteria developed from a separate training set of OCT images. Panel results were compared to histological diagnoses assigned by a blinded pathologist. Results: At the final imaging timepoint, 95% of adenomas and 23% of gastrointestinal neoplasias (38% protruding GINs and 9% non-protruding GINs) were correctly diagnosed. The panel identified 68% of disease foci (95% adenoma, 76% protruding GINs and 13% non-protruding GINs). Over the OCT imaging timepoints, disease progression followed a typical succession, with normal or GIN preceding adenoma. Conclusions: Endoscopic UHR OCT enabled accurate diagnosis of adenomas, identification of protruding GIN and non-destructive visualization of CRC progression, providing a tool for cancer research in animal models.

KW - Animal models for carcinogenesis

KW - Azoxymethane treated mouse model of colorectal neoplasm

KW - Colorectal adenoma

KW - Endoscopic imaging

KW - Gastrointestinal cancer

KW - Gastrointestinal intraepithelial neoplasia

KW - Imaging of tumor progression

UR - http://www.scopus.com/inward/record.url?scp=42149084927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149084927&partnerID=8YFLogxK

M3 - Article

C2 - 17986850

AN - SCOPUS:42149084927

VL - 6

SP - 1753

EP - 1762

JO - Cancer Biology and Therapy

JF - Cancer Biology and Therapy

SN - 1538-4047

IS - 11

ER -