Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography

Lida P. Hariri, Ziping Qiu, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Natalia Ignatenko, Boris Považay, Boris Hermann, Harald Sattmann, James McNally, Angelika Unterhuber, Wolfgang Drexler, Jennifer K Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be commissioned for small diameter endoscopic applications for imaging mouse models of colorectal cancer. In this study, we utilized ultrahigh resolution OCT (UHR OCT) to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC, monitor the progression of neoplastic transformations, and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22, and 26 weeks post AOM treatment) using a prototype 2.0 mm diameter UHR OCT endoscope-based system that achieved resolutions of 3.2 urn axial and 4.4 urn lateral. Histological samples were obtained at the final imaging timepoint serving as the gold standard. Results: Gross and histological assessment of the excised colonie tissue revealed at least one tumor in all 13 AOM treated mice, with most mice developing multiple tumors. In the corresponding OCT images, a progression from healthy thin mucosa to adenoma appearing as large, structurally disorganized masses was visualized over the imaging time points correlating to the locations of the grossly visualized tumors. Conclusions: This study indicates that UHR OCT enables accurate identification of disease and non-destructive visualization of CRC progression in the lower colon of mice.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6432
DOIs
StatePublished - 2007
EventEndoscopic Microscopy II - San Jose, CA, United States
Duration: Jan 21 2007Jan 23 2007

Other

OtherEndoscopic Microscopy II
CountryUnited States
CitySan Jose, CA
Period1/21/071/23/07

Fingerprint

Endoscopy
Optical tomography
Imaging techniques
Tumors
Design of experiments
Visualization
Tissue

Keywords

  • Animal models
  • Colorectal disease
  • Gastrointestinal disease
  • Gastrointestinal tract
  • Mouse anatomy
  • OCT

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Hariri, L. P., Qiu, Z., Tumlinson, A. R., Besselsen, D. G., Gerner, E. W., Ignatenko, N., ... Barton, J. K. (2007). Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6432). [643208] https://doi.org/10.1117/12.699249

Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. / Hariri, Lida P.; Qiu, Ziping; Tumlinson, Alexandre R.; Besselsen, David G.; Gerner, Eugene W.; Ignatenko, Natalia; Považay, Boris; Hermann, Boris; Sattmann, Harald; McNally, James; Unterhuber, Angelika; Drexler, Wolfgang; Barton, Jennifer K.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6432 2007. 643208.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hariri, LP, Qiu, Z, Tumlinson, AR, Besselsen, DG, Gerner, EW, Ignatenko, N, Považay, B, Hermann, B, Sattmann, H, McNally, J, Unterhuber, A, Drexler, W & Barton, JK 2007, Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6432, 643208, Endoscopic Microscopy II, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.699249
Hariri LP, Qiu Z, Tumlinson AR, Besselsen DG, Gerner EW, Ignatenko N et al. Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6432. 2007. 643208 https://doi.org/10.1117/12.699249
Hariri, Lida P. ; Qiu, Ziping ; Tumlinson, Alexandre R. ; Besselsen, David G. ; Gerner, Eugene W. ; Ignatenko, Natalia ; Považay, Boris ; Hermann, Boris ; Sattmann, Harald ; McNally, James ; Unterhuber, Angelika ; Drexler, Wolfgang ; Barton, Jennifer K. / Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6432 2007.
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abstract = "Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be commissioned for small diameter endoscopic applications for imaging mouse models of colorectal cancer. In this study, we utilized ultrahigh resolution OCT (UHR OCT) to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC, monitor the progression of neoplastic transformations, and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22, and 26 weeks post AOM treatment) using a prototype 2.0 mm diameter UHR OCT endoscope-based system that achieved resolutions of 3.2 urn axial and 4.4 urn lateral. Histological samples were obtained at the final imaging timepoint serving as the gold standard. Results: Gross and histological assessment of the excised colonie tissue revealed at least one tumor in all 13 AOM treated mice, with most mice developing multiple tumors. In the corresponding OCT images, a progression from healthy thin mucosa to adenoma appearing as large, structurally disorganized masses was visualized over the imaging time points correlating to the locations of the grossly visualized tumors. Conclusions: This study indicates that UHR OCT enables accurate identification of disease and non-destructive visualization of CRC progression in the lower colon of mice.",
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AU - Gerner, Eugene W.

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AU - Považay, Boris

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