Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography

Lida P. Hariri, Ziping Qiu, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Natalia Ignatenko, Boris Považay, Boris Hermann, Harald Sattmann, James McNally, Angelika Unterhuber, Wolfgang Drexler, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be commissioned for small diameter endoscopic applications for imaging mouse models of colorectal cancer. In this study, we utilized ultrahigh resolution OCT (UHR OCT) to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC, monitor the progression of neoplastic transformations, and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22, and 26 weeks post AOM treatment) using a prototype 2.0 mm diameter UHR OCT endoscope-based system that achieved resolutions of 3.2 urn axial and 4.4 urn lateral. Histological samples were obtained at the final imaging timepoint serving as the gold standard. Results: Gross and histological assessment of the excised colonie tissue revealed at least one tumor in all 13 AOM treated mice, with most mice developing multiple tumors. In the corresponding OCT images, a progression from healthy thin mucosa to adenoma appearing as large, structurally disorganized masses was visualized over the imaging time points correlating to the locations of the grossly visualized tumors. Conclusions: This study indicates that UHR OCT enables accurate identification of disease and non-destructive visualization of CRC progression in the lower colon of mice.

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy II
DOIs
StatePublished - Apr 30 2007
EventEndoscopic Microscopy II - San Jose, CA, United States
Duration: Jan 21 2007Jan 23 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6432
ISSN (Print)1605-7422

Other

OtherEndoscopic Microscopy II
CountryUnited States
CitySan Jose, CA
Period1/21/071/23/07

Keywords

  • Animal models
  • Colorectal disease
  • Gastrointestinal disease
  • Gastrointestinal tract
  • Mouse anatomy
  • OCT

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography'. Together they form a unique fingerprint.

  • Cite this

    Hariri, L. P., Qiu, Z., Tumlinson, A. R., Besselsen, D. G., Gerner, E. W., Ignatenko, N., Považay, B., Hermann, B., Sattmann, H., McNally, J., Unterhuber, A., Drexler, W., & Barton, J. K. (2007). Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography. In Endoscopic Microscopy II [643208] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6432). https://doi.org/10.1117/12.699249