Serotonin immunoreactivity in the optic lobes of the sphinx moth Manduca sexta and colocalization with FMRFamide and SCP(B) immunoreactivity

U. Homberg, John G Hildebrand

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46 Citations (Scopus)

Abstract

In the optic lobes (OLs) of the sphinx moth Manduca sexta, 300-350 neurons per hemisphere are immunoreactive with an antiserotonin antiserum. Two groups of weakly serotonin-immunoreactive cells (OL1) appear to be amacrine cells of the medulla, whereas more intensely immunoreactive cells (OL2) are probably centrifugal neurons that innervate the lobula, medulla, and lamina, as well as the superior protocerebrum. At least one other OL2 cells is a local optic-lobe interneuron with arborizations in the dorsal medulla and lobula. The serotonin-immunoreactive cells are also immunoreactive with an antiserum against Drosophila melanogaster DOPA decarboxylase. All OL2 cells, but not the OL1 cells, are furthermore immunoreactive with an anti-FMRFamide antiserum and an anti-SCP(B) antiserum. This suggests that neuropeptides related or identical to FMRFamide and SCP(B) are colocalized and may serve as cotransmitters with serotonin in OL2 optic-lobe interneurons.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalJournal of Comparative Neurology
Volume288
Issue number2
StatePublished - 1989

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FMRFamide
Manduca
Moths
Serotonin
Immune Sera
Interneurons
Amacrine Cells
Neurons
Carboxy-Lyases
Neuropeptides
Drosophila melanogaster

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Serotonin immunoreactivity in the optic lobes of the sphinx moth Manduca sexta and colocalization with FMRFamide and SCP(B) immunoreactivity",
abstract = "In the optic lobes (OLs) of the sphinx moth Manduca sexta, 300-350 neurons per hemisphere are immunoreactive with an antiserotonin antiserum. Two groups of weakly serotonin-immunoreactive cells (OL1) appear to be amacrine cells of the medulla, whereas more intensely immunoreactive cells (OL2) are probably centrifugal neurons that innervate the lobula, medulla, and lamina, as well as the superior protocerebrum. At least one other OL2 cells is a local optic-lobe interneuron with arborizations in the dorsal medulla and lobula. The serotonin-immunoreactive cells are also immunoreactive with an antiserum against Drosophila melanogaster DOPA decarboxylase. All OL2 cells, but not the OL1 cells, are furthermore immunoreactive with an anti-FMRFamide antiserum and an anti-SCP(B) antiserum. This suggests that neuropeptides related or identical to FMRFamide and SCP(B) are colocalized and may serve as cotransmitters with serotonin in OL2 optic-lobe interneurons.",
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T1 - Serotonin immunoreactivity in the optic lobes of the sphinx moth Manduca sexta and colocalization with FMRFamide and SCP(B) immunoreactivity

AU - Homberg, U.

AU - Hildebrand, John G

PY - 1989

Y1 - 1989

N2 - In the optic lobes (OLs) of the sphinx moth Manduca sexta, 300-350 neurons per hemisphere are immunoreactive with an antiserotonin antiserum. Two groups of weakly serotonin-immunoreactive cells (OL1) appear to be amacrine cells of the medulla, whereas more intensely immunoreactive cells (OL2) are probably centrifugal neurons that innervate the lobula, medulla, and lamina, as well as the superior protocerebrum. At least one other OL2 cells is a local optic-lobe interneuron with arborizations in the dorsal medulla and lobula. The serotonin-immunoreactive cells are also immunoreactive with an antiserum against Drosophila melanogaster DOPA decarboxylase. All OL2 cells, but not the OL1 cells, are furthermore immunoreactive with an anti-FMRFamide antiserum and an anti-SCP(B) antiserum. This suggests that neuropeptides related or identical to FMRFamide and SCP(B) are colocalized and may serve as cotransmitters with serotonin in OL2 optic-lobe interneurons.

AB - In the optic lobes (OLs) of the sphinx moth Manduca sexta, 300-350 neurons per hemisphere are immunoreactive with an antiserotonin antiserum. Two groups of weakly serotonin-immunoreactive cells (OL1) appear to be amacrine cells of the medulla, whereas more intensely immunoreactive cells (OL2) are probably centrifugal neurons that innervate the lobula, medulla, and lamina, as well as the superior protocerebrum. At least one other OL2 cells is a local optic-lobe interneuron with arborizations in the dorsal medulla and lobula. The serotonin-immunoreactive cells are also immunoreactive with an antiserum against Drosophila melanogaster DOPA decarboxylase. All OL2 cells, but not the OL1 cells, are furthermore immunoreactive with an anti-FMRFamide antiserum and an anti-SCP(B) antiserum. This suggests that neuropeptides related or identical to FMRFamide and SCP(B) are colocalized and may serve as cotransmitters with serotonin in OL2 optic-lobe interneurons.

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