Serotonin mediates CA1 spine density but is not crucial for ovarian steroid regulation of synaptic plasticity in the adult rat dorsal hippocampus

Stephen E. Alves, Elena Hoskin, Susan J. Lee, Wayne G. Brake, Deveroux Ferguson, Victoria Luine, Patrick B. Allen, Paul Greengard, Bruce S. McEwen

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The activity of the serotonin (5-hydroxytryptamine, 5-HT) system is sensitive to estradiol and progesterone. During the ovarian cycle, dendritic spines on CA1 pyramidal neurons of the dorsal hippocampus are increased by estradiol and later decreased by progesterone. We sought to determine whether 5-HT is involved in maintaining CA1 spine density and/or in steroid regulation of synaptic plasticity in dorsal hippocampus. Ovariectomized rats were treated (sc) over 10 days with the tryptophan hydroxylase inhibitor parachlorophenylalanine (pCPA) to deplete 5-HT, followed by estradiol benzoate on days 10 and 11. A subset of animals received progesterone on day 12. The day after the last treatment, rats were perfused and brains were processed for Golgi impregnation. Separate groups were processed for radioimmunocytochemistry (RICC) for the spine- associated protein, spinophilin, or high-performance liquid chromatography (HPLC) for monoamine analysis. Golgi and RICC data indicate that CA1 apical spine density was significantly decreased by pCPA (17-20%). Estradiol increased spine density in both saline- and pCPA-treated rats compared to respective controls (30%); however, pCPA animals maintained significantly fewer spines. No differences in spine densities were observed between saline- and pCPA-treated rats given estradiol and progesterone. Depletion of 5-HT by pCPA was confirmed in the CA1 (-90%) and dorsal raphe (-80%) by HPLC analysis. While 5-HT depletion was associated with a 57% decrease in CA1 norepinephrine (NE), there was no difference in dorsal raphe NE. Thus, whereas 5-HT is involved in maintaining spine density in the adult female rat CA1, it is not crucial for steroid-mediated plasticity. 5-HT-regulated spines/synapses may represent distinct populations from those modulated by estradiol and progesterone in dorsal hippocampus.

Original languageEnglish (US)
Pages (from-to)143-151
Number of pages9
JournalSynapse
Volume45
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Dendritic spines
  • Estradiol
  • Norepinephrine
  • Plasticity
  • Progesterone

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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    Alves, S. E., Hoskin, E., Lee, S. J., Brake, W. G., Ferguson, D., Luine, V., Allen, P. B., Greengard, P., & McEwen, B. S. (2002). Serotonin mediates CA1 spine density but is not crucial for ovarian steroid regulation of synaptic plasticity in the adult rat dorsal hippocampus. Synapse, 45(2), 143-151. https://doi.org/10.1002/syn.10093