Serum levels and urine detection of Centruroides sculpturatus venom in significantly envenomated patients

Peter Chase, Leslie Boyer-Hassen, Jude Mcnally, H. L. Vazquez, Andreas A. Theodorou, Frank Gardner Walter, Alejandro Alagon

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Introduction. Envenomation by Centruroides sculpturatus can cause systemic signs and symptoms requiring treatment. The toxicokinetics of C. sculpturatus venom has not been described. Methods. Venom components were separated for cross-reactivity testing. Serum and urine collected from three patients envenomated by C. sculpturatus had venom levels determined by sandwich enzyme-linked immunosorbent assay (ELISA). Results. Western blot analysis indicated recognition of C. sculpturatus venom by Alacramyn®, an equine F(ab′)2 antivenom developed against Centruroides scorpion venoms, including C. sculpturatus. Serum venom levels in ng/mL with post-envenomation times in minutes (min) were as follows: 85-year-old woman = 8.2 (∼150), 2.8 (515), 1.6 (1,200); 14-month-old girl = 29.7 (∼50), 5.0 (729); 3-year-old girl = 11.1 (∼313), urine venom level of 9.0 (∼490). Conclusion. There is sufficient venom cross-antigenicity among different Centruroides species to allow this ELISA technique with Alacramyn® to determine serum and urine C. sculpturatus venom concentrations in envenomated patients.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalClinical Toxicology
Volume47
Issue number1
DOIs
StatePublished - Feb 1 2009

Keywords

  • Antivenom
  • Centruroides sculpturatus
  • Envenomation
  • Scorpion

ASJC Scopus subject areas

  • Toxicology

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