Sevoflurane is biotransformed by guinea pig liver slices but causes minimal cytotoxicity

H. N. Ghantous, J. Fernando, A. J. Gandolfi, K. Brendel

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Guinea pig liver slices were used to evaluate the biotransformation and hepatotoxic potential of sevoflurane. Precision-cut liver slices (250-300 μm thick) were incubated in sealed roller vials in buffer at 37°C under 95% O2. Sevoflurane was added to produce 0.9 or 2.1 mM medium concentrations. After incubation (6-24 h), the intracellular K+ content and protein synthesis were determined, along with the defluorination of sevoflurane. Isoflurane was included for comparative purposes. Sevoflurane (2.1 mM) and isoflurane (2.3 mM) had no effect on slice K+ content, but both anesthetics depressed protein synthesis. The biotransformation of sevoflurane was maximal at 95% O2, with threefold more F- produced from sevoflurane than isoflurane. Sevoflurane appears to have a minimal effect on the guinea pig liver slices, which is consistent with in vivo studies in which minimal or no hepatotoxicity has been observed.

Original languageEnglish (US)
Pages (from-to)436-440
Number of pages5
JournalAnesthesia and analgesia
Volume75
Issue number3
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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