Shared Binding Sites in Lepidoptera for Bacillus thuringiensis Cry1Ja and Cry1A Toxins

Salvador Herrero, Joel González-Cabrera, Bruce E Tabashnik, Juan Ferré

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bacillus thuringiensis toxins act by binding to specific target sites in the insect midgut epithelial membrane. The best-known mechanism of resistance to B. thuringiensis toxins is reduced binding to target sites. Because alteration of a binding site shared by several toxins may cause resistance to all of them, knowledge of which toxins share binding sites is useful for predicting cross-resistance. Conversely, cross-resistance among toxins suggests that the toxins share a binding site. At least two strains of diamondback moth (Plutella xylostella) with resistance to Cry1A toxins and reduced binding of CrylA toxins have strong cross-resistance to Cry1Ja. Thus, we hypothesized that Cry1Ja shares binding sites with Cry1A toxins. We tested this hypothesis in six moth and butterfly species, each from a different family: Cacyreus marshalli (Lycaenidae), Lobesia botrana (Tortricidae), Manduca sexta (Sphingidae), Pectinophora gossypiella (Gelechiidae), P. xylostella (Plutellidae), and Spodoptera exigua (Noctuidae). Although the extent of competition varied among species, experiments with biotinylated Cry1Ja and radiolabeled Cry1Ac showed that Cry1Ja and Cry1Ac competed for binding sites in all six species. A recent report also indicates shared binding sites for Cry1Ja and CrylA toxins in Heliothis virescens (Noctuidae). Thus, shared binding sites for Cry1Ja and Cry1A occur in all lepidopteran species tested so far.

Original languageEnglish (US)
Pages (from-to)5729-5734
Number of pages6
JournalApplied and Environmental Microbiology
Volume67
Issue number12
DOIs
StatePublished - Dec 2001

Fingerprint

Bacillus thuringiensis
Lepidoptera
toxin
binding sites
toxins
Binding Sites
cross resistance
Plutella xylostella
Moths
Noctuidae
Manduca
moth
Spodoptera
Butterflies
Lobesia botrana
Pectinophora gossypiella
Sphingidae
Plutellidae
Insects
Lycaenidae

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Shared Binding Sites in Lepidoptera for Bacillus thuringiensis Cry1Ja and Cry1A Toxins. / Herrero, Salvador; González-Cabrera, Joel; Tabashnik, Bruce E; Ferré, Juan.

In: Applied and Environmental Microbiology, Vol. 67, No. 12, 12.2001, p. 5729-5734.

Research output: Contribution to journalArticle

Herrero, Salvador ; González-Cabrera, Joel ; Tabashnik, Bruce E ; Ferré, Juan. / Shared Binding Sites in Lepidoptera for Bacillus thuringiensis Cry1Ja and Cry1A Toxins. In: Applied and Environmental Microbiology. 2001 ; Vol. 67, No. 12. pp. 5729-5734.
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