Shoot organogenesis from petiole explants in the aquatic plant Nymphoides indica

Matthew A. Jenks, Michael E. Kane, Dennis B. Mcconnell

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A protocol for rapid shoot organogenesis from petiole explants of the ornamental aquatic plant Nymphoides indica L. Thwaites O. Kuntze was developed for use in future mutation breeding and cultivar selection studies. Optimum culture conditions for shoot organogenesis were determined. Effects of factorial combinations of 2-iP, BA or kinetin (0-25 μM) in factorial combination with IAA or NAA (0-25 μM) were examined. On the basis of regeneration frequency (80%) and adventitious shoot number (11.5 shoots per explant), most efficient shoot organogenesis occurred on petiole explants cultured on a basal medium consisting of full-strength MS inorganic salts, 0.56 mM myo-inositol, 1.2 μM thiamine-HCl, 116.8 mM sucrose supplemented with 10 μM BA and 20 μM IAA and solidified with 0.8% TC agar. Formation of adventitious shoots by direct and indirect shoot organogenesis from the same explant was verified by histological sectioning. With the exception of variegated leaf production on a single adventitious shoot produced in the presence of 25 μM kinetin and 15 μM NAA, no visible phenotypic abnormalities were observed in vitro in any of the shoots generated. Solid achlorophyllous adventitious shoots were recovered following culture of this variegated leaf tissue. Plantlets were easily acclimatized to ex vitro conditions.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalPlant Cell, Tissue and Organ Culture
Volume63
Issue number1
DOIs
StatePublished - Dec 1 2000
Externally publishedYes

Keywords

  • Adventitious shoot formation
  • Growth regulators
  • Tissue culture

ASJC Scopus subject areas

  • Horticulture

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