Short-term pulmonary response to inhaled JP-8 jet fuel aerosol in mice

R. F. Robledo, R. S. Young, Robert Clark Lantz, M. L. Witten

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

B6.A.D. (Ahr(d)/Nat(s)) mice were utilized to investigate the short-term pulmonary response to JP-8 jet fuel (JP-8) aerosol inhalation. Mice were nose-only exposed to atmospheres of 0 to 118 mg/m3 for 1 h/d over a period of 7 days to further test the hypothesis that JP-8 concentrations below the permissible exposure level (PEL) of 350 mg/m3 will induce lung injury. At 24 to 30 hours after the final exposure, pulmonary function and respiratory permeability were measured on anesthetized mice and then randomly assigned for bronchoalveolar lavage or histopathology. Bronchoalveolar lavage fluid (BALF) was analyzed for total protein, lactic dehydrogenase (LDH), N-acetyl-β-D-glucosaminidase (NAG), and cytology. Respiratory permeability increases were observed following doses of 48 and 118 mg/m3 and were supported by concomitant BALF increases in total protein and LDH. Conversely, NAG and alveolar macrophage levels decreased following the same exposure concentrations. Morphological lung injury was characterized by the targeting of bronchiolar epithelium and consisted of perivascular edema, Clara cell vacuolization, and necrosis. Alveolar injury included sporadic pulmonary edema, intra-alveolar hemorrhage, and alterations in type II epithelial cells. These results indicate that repeated inhalation of aerosolized JP-8 induces physiological, biochemical, cellular, and morphological lung injury. This study also provides evidence for the reevaluation of the 350 mg/m3 PEL for more volatile petroleum distillates with regard to respirable aerosols.

Original languageEnglish (US)
Pages (from-to)656-663
Number of pages8
JournalToxicologic Pathology
Volume28
Issue number5
StatePublished - 2000

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Hexosaminidases
Lung Injury
Aerosols
Oxidoreductases
Bronchoalveolar Lavage Fluid
Cytology
Petroleum distillates
Lung
Inhalation
Fluids
Permeability
Milk
Proteins
Petroleum
Alveolar Macrophages
Bronchoalveolar Lavage
Pulmonary Edema
Atmosphere
Nose
Cell Biology

Keywords

  • Tc-labeled diethylenetriaminepentaacetic acid (Tc-DTPA)
  • Bronchoalveolar lavage fluid (BALF)
  • Lung injury
  • Morphology
  • Permissable exposure level (PEL)
  • Petroleum hydrocarbons

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pathology and Forensic Medicine
  • Toxicology

Cite this

Robledo, R. F., Young, R. S., Lantz, R. C., & Witten, M. L. (2000). Short-term pulmonary response to inhaled JP-8 jet fuel aerosol in mice. Toxicologic Pathology, 28(5), 656-663.

Short-term pulmonary response to inhaled JP-8 jet fuel aerosol in mice. / Robledo, R. F.; Young, R. S.; Lantz, Robert Clark; Witten, M. L.

In: Toxicologic Pathology, Vol. 28, No. 5, 2000, p. 656-663.

Research output: Contribution to journalArticle

Robledo, RF, Young, RS, Lantz, RC & Witten, ML 2000, 'Short-term pulmonary response to inhaled JP-8 jet fuel aerosol in mice', Toxicologic Pathology, vol. 28, no. 5, pp. 656-663.
Robledo, R. F. ; Young, R. S. ; Lantz, Robert Clark ; Witten, M. L. / Short-term pulmonary response to inhaled JP-8 jet fuel aerosol in mice. In: Toxicologic Pathology. 2000 ; Vol. 28, No. 5. pp. 656-663.
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