Sigma factor RpoN (σ54) regulates pilE transcription in commensal Neisseria elongata

María A. Rendón, Alyson M. Hockenberry, Steven A. Mcmanus, Magdalene "Maggie" So

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human-adapted Neisseria includes two pathogens, Neisseria gonorrhoeae and Neisseria meningitidis, and at least 13 species of commensals that colonize many of the same niches as the pathogens. The Type IV pilus plays an important role in the biology of pathogenic Neisseria. In these species, Sigma factor RpoD (σ70), Integration Host Factor, and repressors RegF and CrgA regulate transcription of pilE, the gene encoding the pilus structural subunit. The Type IV pilus is also a strictly conserved trait in commensal Neisseria. We present evidence that a different mechanism regulates pilE transcription in commensals. Using Neisseria elongata as a model, we show that Sigma factor RpoN (σ54), Integration Host Factor, and an activator we name Npa regulate pilE transcription. Taken in context with previous reports, our findings indicate pilE regulation switched from an RpoN- to an RpoD-dependent mechanism as pathogenic Neisseria diverged from commensals during evolution. Our findings have implications for the timing of Tfp expression and Tfp-mediated host cell interactions in these two groups of bacteria.

Original languageEnglish (US)
Pages (from-to)103-113
Number of pages11
JournalMolecular Microbiology
Volume90
Issue number1
DOIs
StatePublished - Oct 2013

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RNA Polymerase Sigma 54
Neisseria elongata
Neisseria
Integration Host Factors
Neisseria meningitidis
Neisseria gonorrhoeae
Cell Communication
Names
Bacteria
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Sigma factor RpoN (σ54) regulates pilE transcription in commensal Neisseria elongata. / Rendón, María A.; Hockenberry, Alyson M.; Mcmanus, Steven A.; So, Magdalene "Maggie".

In: Molecular Microbiology, Vol. 90, No. 1, 10.2013, p. 103-113.

Research output: Contribution to journalArticle

Rendón, María A. ; Hockenberry, Alyson M. ; Mcmanus, Steven A. ; So, Magdalene "Maggie". / Sigma factor RpoN (σ54) regulates pilE transcription in commensal Neisseria elongata. In: Molecular Microbiology. 2013 ; Vol. 90, No. 1. pp. 103-113.
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