Signaling to a B-cell clone by Ek, but not Ak, does not reflect alteration of Ak genes

Gail Abendroth Bishop, Minnie S. McMillan, Geoffrey Haughton, Jeffrey A Frelinger

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mouse B-cell clone, CH12.LX (Iak, Ly-1+, μ+, δ+), can be induced to differentiate and secrete antibody in an antigen-specific, H-2-restricted manner. Induction requires two signals. One must be provided by the binding of specific antigen to the membrane IgM; the other is delivered by the binding of Ek-specific T-cell hybridomas to the Ek molecules of CH12.LX (Bishop and Haughton 1986). Previous studies demonstrated that Ek-specific monoclonal antibodies (mAbs) could substitute for T cells in delivering the second differentiative signal (Bishop and Haughton 1986). Although CH12.LX cells present Ak to Ak-restricted or alloreactive T-helper cells, neither T cells nor mAbs specific for Ak induce differentiation (Bishop and Haughton 1986). However, since the Akspecifc mAbs tested previously were β-chain-specific and the Ia epitope specificity of the T cells used was unknown, it is possible that the differentiative signal delivered to the CH12.LX class 11 molecule is chain-specific. Here we report the effects of ten additional Iak-specific mAbs upon the differentiation of CH12.LX. In addition, a cl)NA library was prepared from CHI 2.LX cells, clones corresponding to the α and β chains of the Ak molecule were isolated, and their nucleotide sequences were determined. Finally, the Ak and Ek molecules of CH12.LX and H-2k spleen cells were compared by two-dimensional gel electrophoresis to examine possible post-translational differences in the Iak molecules of CH12.LX.

Original languageEnglish (US)
Pages (from-to)184-192
Number of pages9
JournalImmunogenetics
Volume28
Issue number3
DOIs
StatePublished - Sep 1988
Externally publishedYes

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B-Lymphocytes
Clone Cells
Monoclonal Antibodies
T-Lymphocytes
Genes
H-2 Antigens
T-Cell Antigen Receptor Specificity
T-Lymphocyte Epitopes
Electrophoresis, Gel, Two-Dimensional
Hybridomas
Helper-Inducer T-Lymphocytes
Libraries
Immunoglobulin M
Spleen
Antigens
Membranes
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Signaling to a B-cell clone by Ek, but not Ak, does not reflect alteration of Ak genes. / Bishop, Gail Abendroth; McMillan, Minnie S.; Haughton, Geoffrey; Frelinger, Jeffrey A.

In: Immunogenetics, Vol. 28, No. 3, 09.1988, p. 184-192.

Research output: Contribution to journalArticle

Bishop, Gail Abendroth ; McMillan, Minnie S. ; Haughton, Geoffrey ; Frelinger, Jeffrey A. / Signaling to a B-cell clone by Ek, but not Ak, does not reflect alteration of Ak genes. In: Immunogenetics. 1988 ; Vol. 28, No. 3. pp. 184-192.
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abstract = "The mouse B-cell clone, CH12.LX (Iak, Ly-1+, μ+, δ+), can be induced to differentiate and secrete antibody in an antigen-specific, H-2-restricted manner. Induction requires two signals. One must be provided by the binding of specific antigen to the membrane IgM; the other is delivered by the binding of Ek-specific T-cell hybridomas to the Ek molecules of CH12.LX (Bishop and Haughton 1986). Previous studies demonstrated that Ek-specific monoclonal antibodies (mAbs) could substitute for T cells in delivering the second differentiative signal (Bishop and Haughton 1986). Although CH12.LX cells present Ak to Ak-restricted or alloreactive T-helper cells, neither T cells nor mAbs specific for Ak induce differentiation (Bishop and Haughton 1986). However, since the Akspecifc mAbs tested previously were β-chain-specific and the Ia epitope specificity of the T cells used was unknown, it is possible that the differentiative signal delivered to the CH12.LX class 11 molecule is chain-specific. Here we report the effects of ten additional Iak-specific mAbs upon the differentiation of CH12.LX. In addition, a cl)NA library was prepared from CHI 2.LX cells, clones corresponding to the α and β chains of the Ak molecule were isolated, and their nucleotide sequences were determined. Finally, the Ak and Ek molecules of CH12.LX and H-2k spleen cells were compared by two-dimensional gel electrophoresis to examine possible post-translational differences in the Iak molecules of CH12.LX.",
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