Signatures of recombination in clonal lineages of the citrus brown spot pathogen, Alternaria alternata sensu lato

Jane E. Stewart, Kalyn A. Thomas, Christopher B. Lawrence, Ha Dang, Barry M Pryor, L. M. Timmer, Tobin L. Peever

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Abstract

Most Alternaria spp. are considered asexual but recent molecular evolution analyses of Alternaria mating-type genes show that the mating locus is under strong purifying selection, indicating a possible role in sexual reproduction. The objective of this study was to determine the mode of reproduction of an Alternaria alternata sensu lato population causing citrus brown spot in central Florida. Mating type of each isolate was determined, and isolates were sequenced at six putatively unlinked loci. Three genetically distinct subpopulations (SH1, SH4A, and SH4B) were identified using network and Bayesian population structure analyses. Results demonstrate that most subpopulations of A. alternata associated with citrus are clonal but some have the ability to extensively recombine through a cryptic sexual cycle or parasexual cycle. Although isolates were sampled in close physical proximity (≈2,500-m2 area), we were able to reject a random mating model using multilocus gametic disequilibrium tests for two subpopulations, SH1 and SH4B, suggesting that these subpopulations were predominantly asexual. However, three recombination events were identified in SH1 and SH4B and localized to individuals of opposite mating type, possibly indicating meiotic recombination. In contrast, in the third subpopulation (SH4A), where only one mating type was present, extensive reticulation was evident in network analyses, and multilocus gametic disequilibrium tests were consistent with recombination. Recombination among isolates of the same mating type suggests that a nonmeiotic mechanism of recombination such as the parasexual cycle may be operating in this subpopulation. The level of gene flow detected among subpopulations does not appear to be sufficient to prevent differentiation, and perhaps future speciation, of these A. alternata subpopulations.

Original languageEnglish (US)
Pages (from-to)741-749
Number of pages9
JournalPhytopathology
Volume103
Issue number7
DOIs
StatePublished - Jul 2013

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Alternaria alternata
Citrus
parasexual cycle
pathogens
Alternaria
loci
random mating
sexual reproduction
gene flow
population structure
testing
mating types
genes

Keywords

  • Diversity
  • Index of association

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Signatures of recombination in clonal lineages of the citrus brown spot pathogen, Alternaria alternata sensu lato. / Stewart, Jane E.; Thomas, Kalyn A.; Lawrence, Christopher B.; Dang, Ha; Pryor, Barry M; Timmer, L. M.; Peever, Tobin L.

In: Phytopathology, Vol. 103, No. 7, 07.2013, p. 741-749.

Research output: Contribution to journalArticle

Stewart, Jane E. ; Thomas, Kalyn A. ; Lawrence, Christopher B. ; Dang, Ha ; Pryor, Barry M ; Timmer, L. M. ; Peever, Tobin L. / Signatures of recombination in clonal lineages of the citrus brown spot pathogen, Alternaria alternata sensu lato. In: Phytopathology. 2013 ; Vol. 103, No. 7. pp. 741-749.
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