SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy

Ryuichi Katayama, Yuzuru Takashima, No Cheol Park, Geon Lim, Won Sup Lee, Hyungbae Moon, Guk Jong Choi, Young Pil Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have characterized a new type STED microscope which combines a high numerical aperture (NA) optical head with a solid immersion lens (SIL), and we call it as SIL-STED microscope. The advantage of a SIL-STED microscope is that its high NA of the SIL makes it superior to a general STED microscope in lateral resolution, thus overcoming the optical diffraction limit at the macromolecular level and enabling advanced super-resolution imaging of cell surface or cell membrane structure and function Do. This study presents the first implementation of higher NA illumination in a STED microscope limiting the fluorescence lateral resolution to about 40 nm. The refractive index of the SIL which is made of material KTaO3 is about 2.23 and 2.20 at a wavelength of 633 nm and 780 nm which are used for excitation and depletion in STED imaging, respectively. Based on the vector diffraction theory, the electric field focused by the SILSTED microscope is numerically calculated so that the numerical results of the point dispersion function of the microscope and the expected resolution could be analyzed. For further investigation, fluorescence imaging of nano size fluorescent beads is fulfilled to show improved performance of the technique.

Original languageEnglish (US)
Title of host publicationOptical Data Storage 2017
Subtitle of host publicationFrom New Materials to New Systems
PublisherSPIE
Volume10384
ISBN (Electronic)9781510612259
DOIs
StatePublished - Jan 1 2017
EventOptical Data Storage 2017: From New Materials to New Systems, ODS 2017 - San Diego, United States
Duration: Aug 6 2017 → …

Other

OtherOptical Data Storage 2017: From New Materials to New Systems, ODS 2017
CountryUnited States
CitySan Diego
Period8/6/17 → …

Keywords

  • Fluorescence microscopy
  • High numerical aperture
  • Solid immersion lens
  • Stimulated emission depletion (STED) microscopy
  • Superresolution

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Katayama, R., Takashima, Y., Park, N. C., Lim, G., Lee, W. S., Moon, H., Choi, G. J., & Park, Y. P. (2017). SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy. In Optical Data Storage 2017: From New Materials to New Systems (Vol. 10384). [103840F] SPIE. https://doi.org/10.1117/12.2275957