Silencing of soybean seed storage proteins results in a rebalanced protein composition preserving seed protein content without major collateral changes in the metabolome and transcriptome

Monica Schmidt, W. Brad Barbazuk, Michael Sandford, Greg May, Zhihong Song, Wenxu Zhou, Basil J. Nikolau, Eliot M Herman

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to the wild type, maturing to similar size and weight, and in developmental ontogeny. The SP- seeds rebalance the proteome, maintaining wild-type levels of protein and storage triglycerides. The SP- soybeans were evaluated with systems biology techniques of proteomics, metabolomics, and transcriptomics using both microarray and next-generation sequencing transcript sequencing (RNA-Seq). Proteomic analysis shows that rebalancing of protein content largely results from the selective increase in the accumulation of only a few proteins. The rebalancing of protein composition occurs with small alterations to the seed's transcriptome and metabolome. The selectivity of the rebalancing was further tested by introgressing into the SP- line a green fluorescent protein (GFP) glycinin allele mimic and quantifying the resulting accumulation of GFP. The GFP accumulation was similar to the parental GFPexpressing line, showing that the GFP glycinin gene mimic does not participate in proteome rebalancing. The results show that soybeans make large adjustments to the proteome during seed filling and compensate for the shortage of major proteins with the increased selective accumulation of other proteins that maintains a normal protein content.

Original languageEnglish (US)
Pages (from-to)330-345
Number of pages16
JournalPlant Physiology
Volume156
Issue number1
DOIs
StatePublished - May 2011
Externally publishedYes

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Seed Storage Proteins
metabolome
Soybean Proteins
seed storage proteins
Metabolome
protein composition
storage proteins
Transcriptome
transcriptome
Seeds
green fluorescent protein
glycinin
protein content
soybeans
proteome
seeds
Green Fluorescent Proteins
Proteins
proteomics
Proteome

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology
  • Medicine(all)

Cite this

Silencing of soybean seed storage proteins results in a rebalanced protein composition preserving seed protein content without major collateral changes in the metabolome and transcriptome. / Schmidt, Monica; Barbazuk, W. Brad; Sandford, Michael; May, Greg; Song, Zhihong; Zhou, Wenxu; Nikolau, Basil J.; Herman, Eliot M.

In: Plant Physiology, Vol. 156, No. 1, 05.2011, p. 330-345.

Research output: Contribution to journalArticle

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