Simian varicella virus infection of rhesus macaques recapitulates essential features of varicella zoster virus infection in humans

Ilhem Messaoudi, Alexander Barron, Mary Wellish, Flora Engelmann, Alfred Legasse, Shannon Planer, Don Gilden, Janko Nikolich-Zugich, Ravi Mahalingam

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Simian varicella virus (SVV), the etiologic agent of naturally occurring varicella in primates, is genetically and antigenically closely related to human varicella zoster virus (VZV). Early attempts to develop a model of VZV pathogenesis and latency in nonhuman primates (NHP) resulted in persistent infection. More recent models successfully produced latency; however, only a minority of monkeys became viremic and seroconverted. Thus, previous NHP models were not ideally suited to analyze the immune response to SVV during acute infection and the transition to latency. Here, we show for the first time that intrabronchial inoculation of rhesus macaques with SVV closely mimics naturally occurring varicella (chickenpox) in humans. Infected monkeys developed varicella and viremia that resolved 21 days after infection. Months later, viral DNA was detected only in ganglia and not in non-ganglionic tissues. Like VZV latency in human ganglia, transcripts corresponding to SVV ORFs 21, 62, 63 and 66, but not ORF 40, were detected by RT-PCR. In addition, as described for VZV, SVV ORF 63 protein was detected in the cytoplasm of neurons in latently infected monkey ganglia by immunohistochemistry. We also present the first in depth analysis of the immune response to SVV. Infected animals produced a strong humoral and cell-mediated immune response to SVV, as assessed by immunohistology, serology and flow cytometry. Intrabronchial inoculation of rhesus macaques with SVV provides a novel model to analyze viral and immunological mechanisms of VZV latency and reactivation.

Original languageEnglish (US)
Article numbere1000657
JournalPLoS Pathogens
Volume5
Issue number11
DOIs
StatePublished - Nov 2009

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Human Herpesvirus 3
Chickenpox
Virus Diseases
Macaca mulatta
Viruses
Virus Latency
Ganglia
Primates
Open Reading Frames
Haplorhini
Infection
Viremia
Viral DNA
Serology
Flow Cytometry
Cytoplasm
Immunohistochemistry

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Messaoudi, I., Barron, A., Wellish, M., Engelmann, F., Legasse, A., Planer, S., ... Mahalingam, R. (2009). Simian varicella virus infection of rhesus macaques recapitulates essential features of varicella zoster virus infection in humans. PLoS Pathogens, 5(11), [e1000657]. https://doi.org/10.1371/journal.ppat.1000657

Simian varicella virus infection of rhesus macaques recapitulates essential features of varicella zoster virus infection in humans. / Messaoudi, Ilhem; Barron, Alexander; Wellish, Mary; Engelmann, Flora; Legasse, Alfred; Planer, Shannon; Gilden, Don; Nikolich-Zugich, Janko; Mahalingam, Ravi.

In: PLoS Pathogens, Vol. 5, No. 11, e1000657, 11.2009.

Research output: Contribution to journalArticle

Messaoudi, Ilhem ; Barron, Alexander ; Wellish, Mary ; Engelmann, Flora ; Legasse, Alfred ; Planer, Shannon ; Gilden, Don ; Nikolich-Zugich, Janko ; Mahalingam, Ravi. / Simian varicella virus infection of rhesus macaques recapitulates essential features of varicella zoster virus infection in humans. In: PLoS Pathogens. 2009 ; Vol. 5, No. 11.
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