Simple apparatus for serial blood sampling in rodents permitting simultaneous measurement of locomotor activity as illustrated with cocaine

Athiwat Hutchaleelaha, Juthamas Sukbuntherng, Michael Mayersohn

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A simple device has been developed for serial venous blood sampling which permits the simultaneous measurement of locomotor activity in the freely moving rat. The device can be easily constructed from routine laboratory material and it does not interfere with the light beams used to measure locomotor activity. The device, in conjunction with an activity cage, has been applied to the combined pharmacokinetic and pharmacodynamic modeling of cocaine. The relationship between the locomotor activity following a single short iv infusion of cocaine (5 mg/kg) and cocaine plasma concentrations can be adequately described by the Sigmoid-E(max) model. Further, the relationship between activity and time can be described by the same model coupled with an effect compartment. These results suggest the applicability of the device in facilitating pharmacokinetic/pharmacodynamic modeling of drugs that affect locomotor activity.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalJournal of Pharmacological and Toxicological Methods
Volume37
Issue number1
DOIs
StatePublished - Feb 1997

Fingerprint

Locomotion
Cocaine
Pharmacodynamics
Rodentia
Blood
Pharmacokinetics
Sampling
Equipment and Supplies
Rats
Drug Design
Sigmoid Colon
Plasmas
Light
Pharmaceutical Preparations

Keywords

  • Blood sampling device
  • Cocaine
  • Locomotor activity
  • Pharmacokinetic/pharmacodynamic modeling

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Simple apparatus for serial blood sampling in rodents permitting simultaneous measurement of locomotor activity as illustrated with cocaine. / Hutchaleelaha, Athiwat; Sukbuntherng, Juthamas; Mayersohn, Michael.

In: Journal of Pharmacological and Toxicological Methods, Vol. 37, No. 1, 02.1997, p. 9-14.

Research output: Contribution to journalArticle

@article{a54042d0dbf6403d976e2306d9ed04ba,
title = "Simple apparatus for serial blood sampling in rodents permitting simultaneous measurement of locomotor activity as illustrated with cocaine",
abstract = "A simple device has been developed for serial venous blood sampling which permits the simultaneous measurement of locomotor activity in the freely moving rat. The device can be easily constructed from routine laboratory material and it does not interfere with the light beams used to measure locomotor activity. The device, in conjunction with an activity cage, has been applied to the combined pharmacokinetic and pharmacodynamic modeling of cocaine. The relationship between the locomotor activity following a single short iv infusion of cocaine (5 mg/kg) and cocaine plasma concentrations can be adequately described by the Sigmoid-E(max) model. Further, the relationship between activity and time can be described by the same model coupled with an effect compartment. These results suggest the applicability of the device in facilitating pharmacokinetic/pharmacodynamic modeling of drugs that affect locomotor activity.",
keywords = "Blood sampling device, Cocaine, Locomotor activity, Pharmacokinetic/pharmacodynamic modeling",
author = "Athiwat Hutchaleelaha and Juthamas Sukbuntherng and Michael Mayersohn",
year = "1997",
month = "2",
doi = "10.1016/S1056-8719(96)00142-6",
language = "English (US)",
volume = "37",
pages = "9--14",
journal = "Journal of Pharmacological and Toxicological Methods",
issn = "1056-8719",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Simple apparatus for serial blood sampling in rodents permitting simultaneous measurement of locomotor activity as illustrated with cocaine

AU - Hutchaleelaha, Athiwat

AU - Sukbuntherng, Juthamas

AU - Mayersohn, Michael

PY - 1997/2

Y1 - 1997/2

N2 - A simple device has been developed for serial venous blood sampling which permits the simultaneous measurement of locomotor activity in the freely moving rat. The device can be easily constructed from routine laboratory material and it does not interfere with the light beams used to measure locomotor activity. The device, in conjunction with an activity cage, has been applied to the combined pharmacokinetic and pharmacodynamic modeling of cocaine. The relationship between the locomotor activity following a single short iv infusion of cocaine (5 mg/kg) and cocaine plasma concentrations can be adequately described by the Sigmoid-E(max) model. Further, the relationship between activity and time can be described by the same model coupled with an effect compartment. These results suggest the applicability of the device in facilitating pharmacokinetic/pharmacodynamic modeling of drugs that affect locomotor activity.

AB - A simple device has been developed for serial venous blood sampling which permits the simultaneous measurement of locomotor activity in the freely moving rat. The device can be easily constructed from routine laboratory material and it does not interfere with the light beams used to measure locomotor activity. The device, in conjunction with an activity cage, has been applied to the combined pharmacokinetic and pharmacodynamic modeling of cocaine. The relationship between the locomotor activity following a single short iv infusion of cocaine (5 mg/kg) and cocaine plasma concentrations can be adequately described by the Sigmoid-E(max) model. Further, the relationship between activity and time can be described by the same model coupled with an effect compartment. These results suggest the applicability of the device in facilitating pharmacokinetic/pharmacodynamic modeling of drugs that affect locomotor activity.

KW - Blood sampling device

KW - Cocaine

KW - Locomotor activity

KW - Pharmacokinetic/pharmacodynamic modeling

UR - http://www.scopus.com/inward/record.url?scp=0030951774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030951774&partnerID=8YFLogxK

U2 - 10.1016/S1056-8719(96)00142-6

DO - 10.1016/S1056-8719(96)00142-6

M3 - Article

C2 - 9086283

AN - SCOPUS:0030951774

VL - 37

SP - 9

EP - 14

JO - Journal of Pharmacological and Toxicological Methods

JF - Journal of Pharmacological and Toxicological Methods

SN - 1056-8719

IS - 1

ER -