Simple apparatus for serial blood sampling in rodents permitting simultaneous measurement of locomotor activity as illustrated with cocaine

Athiwat Hutchaleelaha, Juthamas Sukbuntherng, Michael Mayersohn

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A simple device has been developed for serial venous blood sampling which permits the simultaneous measurement of locomotor activity in the freely moving rat. The device can be easily constructed from routine laboratory material and it does not interfere with the light beams used to measure locomotor activity. The device, in conjunction with an activity cage, has been applied to the combined pharmacokinetic and pharmacodynamic modeling of cocaine. The relationship between the locomotor activity following a single short iv infusion of cocaine (5 mg/kg) and cocaine plasma concentrations can be adequately described by the Sigmoid-E(max) model. Further, the relationship between activity and time can be described by the same model coupled with an effect compartment. These results suggest the applicability of the device in facilitating pharmacokinetic/pharmacodynamic modeling of drugs that affect locomotor activity.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalJournal of Pharmacological and Toxicological Methods
Volume37
Issue number1
DOIs
StatePublished - Feb 1997

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Keywords

  • Blood sampling device
  • Cocaine
  • Locomotor activity
  • Pharmacokinetic/pharmacodynamic modeling

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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