Simulation of ischemic reperfusion in endothelial cell culture increases apoptosis

C. Holleyman, Douglas F Larson, K. Hunter

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The endothelial layer of the myocardial vasculature serves as an important protective barrier between blood and myocardium. Ischemic reperfusion (I/R) of the endothelium has been shown to initiate a series of events that leads to ischemic reperfusion injury in the heart. At the onset of ischemic reperfusion, endothelial cells initiate apoptosis, a process whereby the cells self-destruct. Ischemic reperfusion was simulated to study its effects on the induction of apoptosis in cultured human endothelial cells (ECV 304). In addition, the cells were treated with nitric oxide (NO) to test its effect on induction of apoptosis. To mimic hypoxia, four ECV 304 cultures were placed in a medium that had been bubbled with pure nitrogen gas for 24 hours. A continuous flow of nitrogen gas was applied to the culture flasks during the course of the 2-hour ischemic period. After 2 hours, the nitrogen was removed from the hypoxic cultures to simulate reperfusion. Exposure to NO was achieved through the NO-donor (±)-S-nitroso-N-acetylpenicillamine (SNAP) at 100 μM. Cell cultures were exposed to hypoxia only, hypoxia and SNAP, and SNAP only. One positive control was established by exposure to staurosporine. A second positive control was established by exposure to a 30-min heat treatment at 43°C. Two cultures were left untreated to serve as negative controls. All cell cultures were incubated for 4 hours. Apoptosis was detected by the binding of annexin V-fluorescein isothiocyanate (annexin V-FITC). In addition, morphologic changes detected by electron microscopy were used. Apoptosis increased in all treated cultures, excluding SNAP only treated cells. It was concluded that I/R may lead to induction of apoptosis.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalJournal of Extra-Corporeal Technology
Volume33
Issue number3
StatePublished - 2001

Fingerprint

S-Nitroso-N-Acetylpenicillamine
Reperfusion
Endothelial Cells
Cell Culture Techniques
Apoptosis
Nitrogen
Nitric Oxide
Gases
Staurosporine
Nitric Oxide Donors
Annexin A5
Reperfusion Injury
Fluorescein
Endothelium
Myocardium
Electron Microscopy
Hot Temperature
Hypoxia

Keywords

  • Annexin V
  • Apoptosis
  • Endothelial cell
  • NFκB
  • Nitric oxide
  • Reperfusion injury

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Simulation of ischemic reperfusion in endothelial cell culture increases apoptosis. / Holleyman, C.; Larson, Douglas F; Hunter, K.

In: Journal of Extra-Corporeal Technology, Vol. 33, No. 3, 2001, p. 175-180.

Research output: Contribution to journalArticle

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