Simulation of stirred tank affinity processes applied to separation of proteins

Rosa Ma Montesinos, Roberto Guzmán, Armando Tejeda-Mansir

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A model, which includes pore diffusion, external film resistance, and finite kinetic rate, was used to mathematically describe a batch affinity adsorption system. The corresponding differential equations system was solved using two numerical methods: the numerical method of lines (NUMOL) and the global (implicit) finite difference method. In each case, simulation studies were conducted to determine the mass-transfer-controlled mechanism. Experimental data from literature describing batch affinity adsorption of immunoglobulin G to protein A-Sepharose was used as a model system. The best fit of the experimental data was obtained with the mass-transfer process controlled by pore diffusion and film resistance, in the simulation studies, using the NUMOL solution. The transport model was used to perform a parametric analysis of the experimental batch system. The influence of both process parameters as well as physical parameters on the affinity adsorption process was investigated.

Original languageEnglish (US)
Pages (from-to)231-243
Number of pages13
JournalInternational Journal of Bio-Chromatography
Volume6
Issue number3
StatePublished - Dec 1 2001
Externally publishedYes

Keywords

  • Adsorption
  • Batch affinity chromatography
  • Biomolecules
  • Mathematical modeling

ASJC Scopus subject areas

  • Biochemistry

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