Simultaneous 2-D analysis of multiple fluorescent probes using multispectral imaging microscopy

Yash S. Sabharwal, Mark F. Hopkins, Ronald M. Lynch, LaTanya Donaldson

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

This research discusses the use of a new multispectral imaging system in fluorescence microscopy applications. We present the results of experiments designed to demonstrate the utility of this instrument for biological investigations. A phantom object was constructed using fluorescent microspheres and imaged to demonstrate the ability of the system to differentiate microscopic structures based on spectral properties. Excellent spectral isolation of subcellular structures, differentially labeled with fluorescent probes, was also obtained in images of neurons and rat sinusoidal epithelial (RSE-1) cells demonstrating that separation of subcellular structures in biological imaging applications is possible with high spatial resolution (up to 0.25 microns) and excellent signal to noise ratio (SNR>30). A final imaging experiment using this device with a confocal micro-endoscope shows the flexibility of the developed instrument when used with different imaging systems. In addition, the results indicate that multispectral investigations of tissue in-vivo may be possible.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages122-128
Number of pages7
Volume3603
StatePublished - 1999
EventProceedings of the 1999 Systems and Technologies for Clinical Diagnostics and Drug Discovery II - San Jose, CA, USA
Duration: Jan 24 1999Jan 25 1999

Other

OtherProceedings of the 1999 Systems and Technologies for Clinical Diagnostics and Drug Discovery II
CitySan Jose, CA, USA
Period1/24/991/25/99

Fingerprint

Imaging systems
Microscopic examination
microscopy
Imaging techniques
endoscopes
Endoscopy
probes
Fluorescence microscopy
Microspheres
neurons
rats
Neurons
Rats
isolation
Signal to noise ratio
flexibility
signal to noise ratios
spatial resolution
Experiments
Tissue

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Sabharwal, Y. S., Hopkins, M. F., Lynch, R. M., & Donaldson, L. (1999). Simultaneous 2-D analysis of multiple fluorescent probes using multispectral imaging microscopy. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3603, pp. 122-128). Society of Photo-Optical Instrumentation Engineers.

Simultaneous 2-D analysis of multiple fluorescent probes using multispectral imaging microscopy. / Sabharwal, Yash S.; Hopkins, Mark F.; Lynch, Ronald M.; Donaldson, LaTanya.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3603 Society of Photo-Optical Instrumentation Engineers, 1999. p. 122-128.

Research output: Chapter in Book/Report/Conference proceedingChapter

Sabharwal, YS, Hopkins, MF, Lynch, RM & Donaldson, L 1999, Simultaneous 2-D analysis of multiple fluorescent probes using multispectral imaging microscopy. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3603, Society of Photo-Optical Instrumentation Engineers, pp. 122-128, Proceedings of the 1999 Systems and Technologies for Clinical Diagnostics and Drug Discovery II, San Jose, CA, USA, 1/24/99.
Sabharwal YS, Hopkins MF, Lynch RM, Donaldson L. Simultaneous 2-D analysis of multiple fluorescent probes using multispectral imaging microscopy. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3603. Society of Photo-Optical Instrumentation Engineers. 1999. p. 122-128
Sabharwal, Yash S. ; Hopkins, Mark F. ; Lynch, Ronald M. ; Donaldson, LaTanya. / Simultaneous 2-D analysis of multiple fluorescent probes using multispectral imaging microscopy. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3603 Society of Photo-Optical Instrumentation Engineers, 1999. pp. 122-128
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