Simultaneous calibration of optical tweezers spring constant and position detector response

Antoine Le Gall, Karen Perronet, David Dulin, André Villing, Philippe Bouyer, Koen Visscher, Nathalie Westbrook

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We demonstrate a fast and direct calibration method for systems using a singlelaser for optical tweezers and particle position detection. The method takes direct advantage of back-focal-plane interferometry measuring not an absolute but a differential position, i.e. the position of the trapped particle relative to the center of the optical tweezers. Therefore, a fast step-wise motion of the optical tweezers yields the impulse response of the trapped particle. Calibration parameters such as the detector's spatial and temporal response and the spring constant of the optical tweezers then follow readily from fitting the measured impulse response.

Original languageEnglish (US)
Pages (from-to)26469-26474
Number of pages6
JournalOptics Express
Volume18
Issue number25
DOIs
StatePublished - Dec 6 2010

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Le Gall, A., Perronet, K., Dulin, D., Villing, A., Bouyer, P., Visscher, K., & Westbrook, N. (2010). Simultaneous calibration of optical tweezers spring constant and position detector response. Optics Express, 18(25), 26469-26474. https://doi.org/10.1364/OE.18.026469