Simultaneous cryosectioning of multiple rodent brains

Tabitha R.F. Green, J. Bryce Ortiz, Jordan L. Harrison, Jonathan Lifshitz, Rachel K. Rowe

Research output: Contribution to journalArticlepeer-review

Abstract

Histology and immunohistochemistry are routine methods of analysis to visualize microscopic anatomy and localize proteins within biological tissue. In neuroscience, as well as a plethora of other scientific fields, these techniques are used. Immunohistochemistry can be done on slide mounted tissue or free-floating sections. Preparing slide-mounted samples is a time intensive process. The following protocol for a technique, called the Megabrain, reduced the time taken to cryosection and mount brain tissue by up to 90% by combining multiple brains into a single frozen block. Furthermore, this technique reduced variability seen between staining rounds, in a large histochemical study. The current technique has been optimized for using rodent brain tissue in downstream immunohistochemical analyses; however, it can be applied to different scientific fields that use cryosectioning.

Original languageEnglish (US)
Article numbere58513
JournalJournal of Visualized Experiments
Volume2018
Issue number139
DOIs
StatePublished - Sep 18 2018

Keywords

  • Cryoprotection
  • Cryosectioning
  • Cryostat
  • Histology
  • Immunohistochemistry
  • Issue 139
  • Neuropathology
  • Neuroscience
  • Neuroscience
  • Rodent

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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