Simultaneous cryosectioning of multiple rodent brains

Tabitha R.F. Green, J. Bryce Ortiz, Jordan L. Harrison, Jonathan Lifshitz, Rachel K. Rowe

Research output: Contribution to journalArticle

Abstract

Histology and immunohistochemistry are routine methods of analysis to visualize microscopic anatomy and localize proteins within biological tissue. In neuroscience, as well as a plethora of other scientific fields, these techniques are used. Immunohistochemistry can be done on slide mounted tissue or free-floating sections. Preparing slide-mounted samples is a time intensive process. The following protocol for a technique, called the Megabrain, reduced the time taken to cryosection and mount brain tissue by up to 90% by combining multiple brains into a single frozen block. Furthermore, this technique reduced variability seen between staining rounds, in a large histochemical study. The current technique has been optimized for using rodent brain tissue in downstream immunohistochemical analyses; however, it can be applied to different scientific fields that use cryosectioning.

Original languageEnglish (US)
Article numbere58513
JournalJournal of Visualized Experiments
Volume2018
Issue number139
DOIs
StatePublished - Sep 18 2018
Externally publishedYes

Keywords

  • Cryoprotection
  • Cryosectioning
  • Cryostat
  • Histology
  • Immunohistochemistry
  • Issue 139
  • Neuropathology
  • Neuroscience
  • Neuroscience
  • Rodent

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Green, T. R. F., Ortiz, J. B., Harrison, J. L., Lifshitz, J., & Rowe, R. K. (2018). Simultaneous cryosectioning of multiple rodent brains. Journal of Visualized Experiments, 2018(139), [e58513]. https://doi.org/10.3791/58513