Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical imaging and MRI have both been used extensively to study tumor microenvironment. The two imaging modalities are complementary and can be used to cross-validate one another for specific measurements. We have developed a modular platform that is capable of doing optical microscopy inside an MRI instrument. To do this, an optical relay system transfers the image to outside of the MR bore to a commercial grade CCD camera. This enables simultaneous optical and MR imaging of the same tissue and thus creates the ideal situation for comparative or complementary studies using both modalities. Initial experiments have been done using GFP labeled prostate cancer cells implanted in mouse dorsal skin fold window chamber. Vascular hemodynamics and vascular permeability were studied using our imaging system. Towards this goal, we developed a dual MR-Optical contrast agent by labeling BSA with both Gd-DTPA and Alexa Fluor. Overall system design and results of these preliminary vascular studies are presented.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7902
DOIs
StatePublished - 2011
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX - San Francisco, CA, United States
Duration: Jan 22 2011Jan 25 2011

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
CountryUnited States
CitySan Francisco, CA
Period1/22/111/25/11

Fingerprint

optical relay systems
hemodynamics
Optical Imaging
CCD cameras
systems engineering
marking
Blood Vessels
mice
grade
Skin
permeability
tumors
platforms
chambers
cancer
microscopy
Imaging techniques
Optical Devices
Magnetic resonance imaging
Gadolinium DTPA

Keywords

  • Dual-Modality
  • Fluorescence
  • MRI
  • Optical Imaging
  • Simultaneous
  • Vascular Permeability
  • Window Chamber

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Salek, M. F., Pagel, M. M., & Gmitro, A. F. (2011). Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7902). [79020G] https://doi.org/10.1117/12.876529

Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber. / Salek, Mir Farrokh; Pagel, Mark "Marty"; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7902 2011. 79020G.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Salek, MF, Pagel, MM & Gmitro, AF 2011, Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7902, 79020G, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.876529
Salek MF, Pagel MM, Gmitro AF. Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7902. 2011. 79020G https://doi.org/10.1117/12.876529
Salek, Mir Farrokh ; Pagel, Mark "Marty" ; Gmitro, Arthur F. / Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7902 2011.
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