Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber

Mir Farrokh Salek, Mark D. Pagel, Arthur F. Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical imaging and MRI have both been used extensively to study tumor microenvironment. The two imaging modalities are complementary and can be used to cross-validate one another for specific measurements. We have developed a modular platform that is capable of doing optical microscopy inside an MRI instrument. To do this, an optical relay system transfers the image to outside of the MR bore to a commercial grade CCD camera. This enables simultaneous optical and MR imaging of the same tissue and thus creates the ideal situation for comparative or complementary studies using both modalities. Initial experiments have been done using GFP labeled prostate cancer cells implanted in mouse dorsal skin fold window chamber. Vascular hemodynamics and vascular permeability were studied using our imaging system. Towards this goal, we developed a dual MR-Optical contrast agent by labeling BSA with both Gd-DTPA and Alexa Fluor. Overall system design and results of these preliminary vascular studies are presented.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
DOIs
StatePublished - Apr 20 2011
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX - San Francisco, CA, United States
Duration: Jan 22 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7902
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
CountryUnited States
CitySan Francisco, CA
Period1/22/111/25/11

Keywords

  • Dual-Modality
  • Fluorescence
  • MRI
  • Optical Imaging
  • Simultaneous
  • Vascular Permeability
  • Window Chamber

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber'. Together they form a unique fingerprint.

  • Cite this

    Salek, M. F., Pagel, M. D., & Gmitro, A. F. (2011). Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX [79020G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7902). https://doi.org/10.1117/12.876529