Simultaneous irradiation and imaging of blood vessels during pulsed laser delivery

Jennifer K Barton, Daniel X. Hammer, T. Joshua Pfefer, David J. Lund, Bruce E. Stuck, A. J. Welch

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background and Objective: Simultaneous irradiation and viewing of 10- 120 μm cutaneous blood vessels were performed to investigate the effects of 2-μs 577-nm dye laser pulses. Study Design/Materials and Methods: A modified scanning laser confocal microscope recorded vessel response to different radiant exposures (J/cm2). Probit analysis determined the 50% probability ('threshold') radiant exposure necessary to cause embolized or partly occluding coagula, coagula causing complete blood flow stoppage, and hemorrhage. Results: A statistically significant difference in the threshold radiant exposure existed for each damage category for blood vessels 10-30 μm in diameter, but not for larger vessels. For vessels over 60 μm, complete flow stoppage was unattainable; increasing laser pulse energy produced hemorrhage. In larger vessels, coagula often were attached to the superficial vessel wall while blood flowed underneath. Monte Carlo optical and finite difference thermal modeling confirmed experimental results. Conclusion: These results provide insight into the role of pulse duration and vessel diameter in the outcome of pulsed dye laser irradiation.

Original languageEnglish (US)
Pages (from-to)236-243
Number of pages8
JournalLasers in Surgery and Medicine
Volume24
Issue number3
DOIs
StatePublished - 1999

Fingerprint

Dye Lasers
Blood Vessels
Lasers
Hemorrhage
Hot Temperature
Skin

Keywords

  • Confocal microscopy
  • Finite difference model
  • Monte Carlo model
  • Port wine stains
  • Pulsed dye laser

ASJC Scopus subject areas

  • Surgery

Cite this

Simultaneous irradiation and imaging of blood vessels during pulsed laser delivery. / Barton, Jennifer K; Hammer, Daniel X.; Pfefer, T. Joshua; Lund, David J.; Stuck, Bruce E.; Welch, A. J.

In: Lasers in Surgery and Medicine, Vol. 24, No. 3, 1999, p. 236-243.

Research output: Contribution to journalArticle

Barton, Jennifer K ; Hammer, Daniel X. ; Pfefer, T. Joshua ; Lund, David J. ; Stuck, Bruce E. ; Welch, A. J. / Simultaneous irradiation and imaging of blood vessels during pulsed laser delivery. In: Lasers in Surgery and Medicine. 1999 ; Vol. 24, No. 3. pp. 236-243.
@article{966ddc225dfa435ca18bf86962b53c01,
title = "Simultaneous irradiation and imaging of blood vessels during pulsed laser delivery",
abstract = "Background and Objective: Simultaneous irradiation and viewing of 10- 120 μm cutaneous blood vessels were performed to investigate the effects of 2-μs 577-nm dye laser pulses. Study Design/Materials and Methods: A modified scanning laser confocal microscope recorded vessel response to different radiant exposures (J/cm2). Probit analysis determined the 50{\%} probability ('threshold') radiant exposure necessary to cause embolized or partly occluding coagula, coagula causing complete blood flow stoppage, and hemorrhage. Results: A statistically significant difference in the threshold radiant exposure existed for each damage category for blood vessels 10-30 μm in diameter, but not for larger vessels. For vessels over 60 μm, complete flow stoppage was unattainable; increasing laser pulse energy produced hemorrhage. In larger vessels, coagula often were attached to the superficial vessel wall while blood flowed underneath. Monte Carlo optical and finite difference thermal modeling confirmed experimental results. Conclusion: These results provide insight into the role of pulse duration and vessel diameter in the outcome of pulsed dye laser irradiation.",
keywords = "Confocal microscopy, Finite difference model, Monte Carlo model, Port wine stains, Pulsed dye laser",
author = "Barton, {Jennifer K} and Hammer, {Daniel X.} and Pfefer, {T. Joshua} and Lund, {David J.} and Stuck, {Bruce E.} and Welch, {A. J.}",
year = "1999",
doi = "10.1002/(SICI)1096-9101(1999)24:3<236::AID-LSM9>3.0.CO;2-I",
language = "English (US)",
volume = "24",
pages = "236--243",
journal = "Lasers in Surgery and Medicine",
issn = "0196-8092",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Simultaneous irradiation and imaging of blood vessels during pulsed laser delivery

AU - Barton, Jennifer K

AU - Hammer, Daniel X.

AU - Pfefer, T. Joshua

AU - Lund, David J.

AU - Stuck, Bruce E.

AU - Welch, A. J.

PY - 1999

Y1 - 1999

N2 - Background and Objective: Simultaneous irradiation and viewing of 10- 120 μm cutaneous blood vessels were performed to investigate the effects of 2-μs 577-nm dye laser pulses. Study Design/Materials and Methods: A modified scanning laser confocal microscope recorded vessel response to different radiant exposures (J/cm2). Probit analysis determined the 50% probability ('threshold') radiant exposure necessary to cause embolized or partly occluding coagula, coagula causing complete blood flow stoppage, and hemorrhage. Results: A statistically significant difference in the threshold radiant exposure existed for each damage category for blood vessels 10-30 μm in diameter, but not for larger vessels. For vessels over 60 μm, complete flow stoppage was unattainable; increasing laser pulse energy produced hemorrhage. In larger vessels, coagula often were attached to the superficial vessel wall while blood flowed underneath. Monte Carlo optical and finite difference thermal modeling confirmed experimental results. Conclusion: These results provide insight into the role of pulse duration and vessel diameter in the outcome of pulsed dye laser irradiation.

AB - Background and Objective: Simultaneous irradiation and viewing of 10- 120 μm cutaneous blood vessels were performed to investigate the effects of 2-μs 577-nm dye laser pulses. Study Design/Materials and Methods: A modified scanning laser confocal microscope recorded vessel response to different radiant exposures (J/cm2). Probit analysis determined the 50% probability ('threshold') radiant exposure necessary to cause embolized or partly occluding coagula, coagula causing complete blood flow stoppage, and hemorrhage. Results: A statistically significant difference in the threshold radiant exposure existed for each damage category for blood vessels 10-30 μm in diameter, but not for larger vessels. For vessels over 60 μm, complete flow stoppage was unattainable; increasing laser pulse energy produced hemorrhage. In larger vessels, coagula often were attached to the superficial vessel wall while blood flowed underneath. Monte Carlo optical and finite difference thermal modeling confirmed experimental results. Conclusion: These results provide insight into the role of pulse duration and vessel diameter in the outcome of pulsed dye laser irradiation.

KW - Confocal microscopy

KW - Finite difference model

KW - Monte Carlo model

KW - Port wine stains

KW - Pulsed dye laser

UR - http://www.scopus.com/inward/record.url?scp=0032905922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032905922&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9101(1999)24:3<236::AID-LSM9>3.0.CO;2-I

DO - 10.1002/(SICI)1096-9101(1999)24:3<236::AID-LSM9>3.0.CO;2-I

M3 - Article

C2 - 10229155

AN - SCOPUS:0032905922

VL - 24

SP - 236

EP - 243

JO - Lasers in Surgery and Medicine

JF - Lasers in Surgery and Medicine

SN - 0196-8092

IS - 3

ER -