Simultaneous multielement-specific detection of a novel glutathione-arsenic-selenium ion [(GS)2AsSe]- by ICP AES after micellar size-exclusion chromatography

J. Gailer, S. Madden, M. F. Burke, M Bonner Denton, H. V. Aposhian

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In order to confirm the solution structure of [(GS)2AsSe]- (GS = glutathione), we have investigated the retention behaviour of a [(GS)2AsSe]-/oxidized glutathione (GSSG) mixture on a Sephadex G-25 (SF) column with Tris buffers (0.1 mol dm-3, pH 8.0) containing various surfactants at concentrations above the critical micellar concentration (CMC): hexadecyltrimethlammonium bromide (HDTAB; 30, 40 and 50 mmol dm-3); dodecyltrimethylammonium bromide (DDTAB; 50 mmol dm-3); and sodium lauryl sulfate (SLS; 50 mmol dm-3). An inductively coupled plasma atomic emission spectrometer (ICP AES) provided simultaneous on-line detection of arsenic, selenium and sulfur in the column effluent. The chromatographic retention behaviour was used to investigate the association of both compounds with the positively charged micelles (HDTAB and DDTAB mobile phases). The relative strength of association with the micelles provided insight into the effective negative charge on [(GS)2AsSe]- and GSSG. The chromatograms obtained with 50 mmol dm-3 HDTAB indicated that two glutathione molecules are associated with the elution of an arsenic-selenium compound. Combined, these chromatographic data strongly support the spectroscopically derived solution structure of [(GS)2AsSe]-. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish (US)
Pages (from-to)355-363
Number of pages9
JournalApplied Organometallic Chemistry
Volume14
Issue number7
DOIs
StatePublished - 2000

Fingerprint

Size exclusion chromatography
Arsenic
Inductively coupled plasma
Selenium
Glutathione
Spectrometers
Ions
Glutathione Disulfide
Micelles
Selenium Compounds
Association reactions
Tromethamine
Bromides
Sulfur
Surface-Active Agents
Sodium Dodecyl Sulfate
Effluents
Molecules

Keywords

  • Chromatography
  • Glutathione-arsenic-selenium ion
  • Micelles

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Inorganic Chemistry

Cite this

Simultaneous multielement-specific detection of a novel glutathione-arsenic-selenium ion [(GS)2AsSe]- by ICP AES after micellar size-exclusion chromatography. / Gailer, J.; Madden, S.; Burke, M. F.; Denton, M Bonner; Aposhian, H. V.

In: Applied Organometallic Chemistry, Vol. 14, No. 7, 2000, p. 355-363.

Research output: Contribution to journalArticle

@article{31e42aff61e84bdea51c0680a9646a2f,
title = "Simultaneous multielement-specific detection of a novel glutathione-arsenic-selenium ion [(GS)2AsSe]- by ICP AES after micellar size-exclusion chromatography",
abstract = "In order to confirm the solution structure of [(GS)2AsSe]- (GS = glutathione), we have investigated the retention behaviour of a [(GS)2AsSe]-/oxidized glutathione (GSSG) mixture on a Sephadex G-25 (SF) column with Tris buffers (0.1 mol dm-3, pH 8.0) containing various surfactants at concentrations above the critical micellar concentration (CMC): hexadecyltrimethlammonium bromide (HDTAB; 30, 40 and 50 mmol dm-3); dodecyltrimethylammonium bromide (DDTAB; 50 mmol dm-3); and sodium lauryl sulfate (SLS; 50 mmol dm-3). An inductively coupled plasma atomic emission spectrometer (ICP AES) provided simultaneous on-line detection of arsenic, selenium and sulfur in the column effluent. The chromatographic retention behaviour was used to investigate the association of both compounds with the positively charged micelles (HDTAB and DDTAB mobile phases). The relative strength of association with the micelles provided insight into the effective negative charge on [(GS)2AsSe]- and GSSG. The chromatograms obtained with 50 mmol dm-3 HDTAB indicated that two glutathione molecules are associated with the elution of an arsenic-selenium compound. Combined, these chromatographic data strongly support the spectroscopically derived solution structure of [(GS)2AsSe]-. Copyright (C) 2000 John Wiley and Sons, Ltd.",
keywords = "Chromatography, Glutathione-arsenic-selenium ion, Micelles",
author = "J. Gailer and S. Madden and Burke, {M. F.} and Denton, {M Bonner} and Aposhian, {H. V.}",
year = "2000",
doi = "10.1002/1099-0739(200007)14:7<355::AID-AOC8>3.0.CO;2-0",
language = "English (US)",
volume = "14",
pages = "355--363",
journal = "Applied Organometallic Chemistry",
issn = "0268-2605",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

TY - JOUR

T1 - Simultaneous multielement-specific detection of a novel glutathione-arsenic-selenium ion [(GS)2AsSe]- by ICP AES after micellar size-exclusion chromatography

AU - Gailer, J.

AU - Madden, S.

AU - Burke, M. F.

AU - Denton, M Bonner

AU - Aposhian, H. V.

PY - 2000

Y1 - 2000

N2 - In order to confirm the solution structure of [(GS)2AsSe]- (GS = glutathione), we have investigated the retention behaviour of a [(GS)2AsSe]-/oxidized glutathione (GSSG) mixture on a Sephadex G-25 (SF) column with Tris buffers (0.1 mol dm-3, pH 8.0) containing various surfactants at concentrations above the critical micellar concentration (CMC): hexadecyltrimethlammonium bromide (HDTAB; 30, 40 and 50 mmol dm-3); dodecyltrimethylammonium bromide (DDTAB; 50 mmol dm-3); and sodium lauryl sulfate (SLS; 50 mmol dm-3). An inductively coupled plasma atomic emission spectrometer (ICP AES) provided simultaneous on-line detection of arsenic, selenium and sulfur in the column effluent. The chromatographic retention behaviour was used to investigate the association of both compounds with the positively charged micelles (HDTAB and DDTAB mobile phases). The relative strength of association with the micelles provided insight into the effective negative charge on [(GS)2AsSe]- and GSSG. The chromatograms obtained with 50 mmol dm-3 HDTAB indicated that two glutathione molecules are associated with the elution of an arsenic-selenium compound. Combined, these chromatographic data strongly support the spectroscopically derived solution structure of [(GS)2AsSe]-. Copyright (C) 2000 John Wiley and Sons, Ltd.

AB - In order to confirm the solution structure of [(GS)2AsSe]- (GS = glutathione), we have investigated the retention behaviour of a [(GS)2AsSe]-/oxidized glutathione (GSSG) mixture on a Sephadex G-25 (SF) column with Tris buffers (0.1 mol dm-3, pH 8.0) containing various surfactants at concentrations above the critical micellar concentration (CMC): hexadecyltrimethlammonium bromide (HDTAB; 30, 40 and 50 mmol dm-3); dodecyltrimethylammonium bromide (DDTAB; 50 mmol dm-3); and sodium lauryl sulfate (SLS; 50 mmol dm-3). An inductively coupled plasma atomic emission spectrometer (ICP AES) provided simultaneous on-line detection of arsenic, selenium and sulfur in the column effluent. The chromatographic retention behaviour was used to investigate the association of both compounds with the positively charged micelles (HDTAB and DDTAB mobile phases). The relative strength of association with the micelles provided insight into the effective negative charge on [(GS)2AsSe]- and GSSG. The chromatograms obtained with 50 mmol dm-3 HDTAB indicated that two glutathione molecules are associated with the elution of an arsenic-selenium compound. Combined, these chromatographic data strongly support the spectroscopically derived solution structure of [(GS)2AsSe]-. Copyright (C) 2000 John Wiley and Sons, Ltd.

KW - Chromatography

KW - Glutathione-arsenic-selenium ion

KW - Micelles

UR - http://www.scopus.com/inward/record.url?scp=0033663723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033663723&partnerID=8YFLogxK

U2 - 10.1002/1099-0739(200007)14:7<355::AID-AOC8>3.0.CO;2-0

DO - 10.1002/1099-0739(200007)14:7<355::AID-AOC8>3.0.CO;2-0

M3 - Article

VL - 14

SP - 355

EP - 363

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

SN - 0268-2605

IS - 7

ER -