Abstract
Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis.
| Original language | English (US) |
|---|---|
| Article number | 036020 |
| Journal | Journal of Biomedical Optics |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2014 |
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Keywords
- fallopian tube
- fluorescence
- holographic imaging
- ovarian cancer
- ovary
- volume holography
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging. / Orsinger, Gabriel V.; Watson, Jennifer M.; Gordon, Michael; Nymeyer, Ariel C.; De Leon, Erich E.; Brownlee, Johnathan W.; Hatch, Kenneth D; Chambers, Setsuko K; Barton, Jennifer K; Kostuk, Raymond K; Romanowski, Marek.
In: Journal of Biomedical Optics, Vol. 19, No. 3, 036020, 2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging
AU - Orsinger, Gabriel V.
AU - Watson, Jennifer M.
AU - Gordon, Michael
AU - Nymeyer, Ariel C.
AU - De Leon, Erich E.
AU - Brownlee, Johnathan W.
AU - Hatch, Kenneth D
AU - Chambers, Setsuko K
AU - Barton, Jennifer K
AU - Kostuk, Raymond K
AU - Romanowski, Marek
PY - 2014
Y1 - 2014
N2 - Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis.
AB - Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis.
KW - fallopian tube
KW - fluorescence
KW - holographic imaging
KW - ovarian cancer
KW - ovary
KW - volume holography
UR - http://www.scopus.com/inward/record.url?scp=84897143635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897143635&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.19.3.036020
DO - 10.1117/1.JBO.19.3.036020
M3 - Article
VL - 19
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 3
M1 - 036020
ER -