Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

Gabriel V. Orsinger, Jennifer M. Watson, Michael Gordon, Ariel C. Nymeyer, Erich E. De Leon, Johnathan W. Brownlee, Kenneth D Hatch, Setsuko K Chambers, Jennifer K Barton, Raymond K Kostuk, Marek Romanowski

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis.

Original languageEnglish (US)
Article number036020
JournalJournal of Biomedical Optics
Volume19
Issue number3
DOIs
StatePublished - 2014

Fingerprint

cancer
Tissue
Imaging techniques
Imaging systems
pathogenesis
abnormalities
surgery
Surgery
Visualization
Fluorescence
tubes
reflectance
fluorescence

Keywords

  • fallopian tube
  • fluorescence
  • holographic imaging
  • ovarian cancer
  • ovary
  • volume holography

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Orsinger, G. V., Watson, J. M., Gordon, M., Nymeyer, A. C., De Leon, E. E., Brownlee, J. W., ... Romanowski, M. (2014). Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging. Journal of Biomedical Optics, 19(3), [036020]. https://doi.org/10.1117/1.JBO.19.3.036020

Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging. / Orsinger, Gabriel V.; Watson, Jennifer M.; Gordon, Michael; Nymeyer, Ariel C.; De Leon, Erich E.; Brownlee, Johnathan W.; Hatch, Kenneth D; Chambers, Setsuko K; Barton, Jennifer K; Kostuk, Raymond K; Romanowski, Marek.

In: Journal of Biomedical Optics, Vol. 19, No. 3, 036020, 2014.

Research output: Contribution to journalArticle

Orsinger, Gabriel V. ; Watson, Jennifer M. ; Gordon, Michael ; Nymeyer, Ariel C. ; De Leon, Erich E. ; Brownlee, Johnathan W. ; Hatch, Kenneth D ; Chambers, Setsuko K ; Barton, Jennifer K ; Kostuk, Raymond K ; Romanowski, Marek. / Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging. In: Journal of Biomedical Optics. 2014 ; Vol. 19, No. 3.
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