Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models

Jennifer K Barton, Shuo Tang, Ryan Lim, Bruce J. Tromberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Three-layer skin-equivalent models (rafts) were created consisting of a collagen/fibroblast layer and an air-exposed keratinocyte layer. Rafts were imaged with a tri-modality microscope including optical coherence (OC), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) channels. Some rafts were stained with Hoechst 33343 or rhodamine 123, and some were exposed to dimethyl sulfoxide (DMSO). OC microscopy revealed signal in cell cytoplasm and nuclear membranes, and a characteristic texture in the collagen/fibroblast layer. TPEF showed signal in cell cytoplasm and from collagen, and stained specimens revealed cell nuclei or mitochondria. There was little SHG in the keratinocyte layer, but strong signal from collagen bundles. Endogenous signals were severely attenuated in DMSO treated rafts; stained samples revealed shrunken and distorted cell structure. OC, TPEF, and SHG can provide complementary and non-destructive information about raft structure and effect of chemical agents.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6627
StatePublished - 2007
EventOptical Coherence Tomography and Coherence Techniques III - Munich, Germany
Duration: Jun 17 2007Jun 19 2007

Other

OtherOptical Coherence Tomography and Coherence Techniques III
CountryGermany
CityMunich
Period6/17/076/19/07

Fingerprint

Collagen
Skin
Microscopic examination
Harmonic generation
Tissue
Dimethyl sulfoxide
Photons
Fluorescence
Fibroblasts
Mitochondria
Microscopes
Textures
Cells
Membranes
Air
Keratinocytes

Keywords

  • Dimethyl sulfoxide
  • Fluorescence imaging
  • Image contrast
  • Multiphoton microscopy
  • Optical coherence tomography
  • Skin

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Barton, J. K., Tang, S., Lim, R., & Tromberg, B. J. (2007). Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6627). [66270X]

Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. / Barton, Jennifer K; Tang, Shuo; Lim, Ryan; Tromberg, Bruce J.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6627 2007. 66270X.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Barton, JK, Tang, S, Lim, R & Tromberg, BJ 2007, Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6627, 66270X, Optical Coherence Tomography and Coherence Techniques III, Munich, Germany, 6/17/07.
Barton JK, Tang S, Lim R, Tromberg BJ. Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6627. 2007. 66270X
Barton, Jennifer K ; Tang, Shuo ; Lim, Ryan ; Tromberg, Bruce J. / Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6627 2007.
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