Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models

Jennifer K. Barton, Shuo Tang, Ryan Lim, Bruce J. Tromberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Three-layer skin-equivalent models (rafts) were created consisting of a collagen/fibroblast layer and an air-exposed keratinocyte layer. Rafts were imaged with a tri-modality microscope including optical coherence (OC), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) channels. Some rafts were stained with Hoechst 33343 or rhodamine 123, and some were exposed to dimethyl sulfoxide (DMSO). OC microscopy revealed signal in cell cytoplasm and nuclear membranes, and a characteristic texture in the collagen/fibroblast layer. TPEF showed signal in cell cytoplasm and from collagen, and stained specimens revealed cell nuclei or mitochondria. There was little SHG in the keratinocyte layer, but strong signal from collagen bundles. Endogenous signals were severely attenuated in DMSO treated rafts; stained samples revealed shrunken and distorted cell structure. OC, TPEF, and SHG can provide complementary and non-destructive information about raft structure and effect of chemical agents.

Original languageEnglish (US)
Title of host publicationOptical Coherence Tomography and Coherence Techniques III
PublisherSPIE
ISBN (Print)0819467715, 9780819467713
DOIs
StatePublished - Jan 1 2007
EventOptical Coherence Tomography and Coherence Techniques III - Munich, Germany
Duration: Jun 17 2007Jun 19 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6627
ISSN (Print)1605-7422

Other

OtherOptical Coherence Tomography and Coherence Techniques III
CountryGermany
CityMunich
Period6/17/076/19/07

Keywords

  • Dimethyl sulfoxide
  • Fluorescence imaging
  • Image contrast
  • Multiphoton microscopy
  • Optical coherence tomography
  • Skin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Barton, J. K., Tang, S., Lim, R., & Tromberg, B. J. (2007). Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. In Optical Coherence Tomography and Coherence Techniques III [66270X] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6627). SPIE. https://doi.org/10.1117/12.728062