Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models

Jennifer K. Barton, Shuo Tangb, Ryan Limb, Bruce J. Tromberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Three-layer skin-equivalent models (rafts) were created consisting of a collagen/fibroblast layer and an airexposed keratinocyte layer. Rafts were imaged with a tri-modality microscope including optical coherence (OC), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) channels. Some rafts were stained with Hoechst 33343 or rhodamine 123, and some were exposed to dimethyl sulfoxide (DMSO). OC microscopy revealed signal in cell cytoplasm and nuclear membranes, and a characteristic texture in the collagen/fibroblast layer. TPEF showed signal in cell cytoplasm and from collagen, and stained specimens revealed cell nuclei or mitochondria. There was little SHG in the keratinocyte layer, but strong signal from collagen bundles. Endogenous signals were severely attenuated in DMSO treated rafts; stained samples revealed shrunken and distorted cell structure. OC, TPEF, and SHG can provide complementary and nondestructive information about raft structure and effect of chemical agents.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2007
PublisherOptical Society of America
ISBN (Print)9780819467713
StatePublished - Jan 1 2007
EventEuropean Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
Duration: Jun 17 2007Jun 17 2007

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2007
CountryGermany
CityMunich
Period6/17/076/17/07

Keywords

  • Dimethyl sulfoxide
  • Fluorescence imaging
  • Image contrast
  • Multiphoton microscopy
  • Optical coherence tomography
  • Skin

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Barton, J. K., Tangb, S., Limb, R., & Tromberg, B. J. (2007). Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models. In European Conference on Biomedical Optics, ECBO 2007 (Optics InfoBase Conference Papers). Optical Society of America.