Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography

Ashok K. Batta, Gerald Salen, Priti Batta, G. Stephen Tint, David S. Alberts, David L. Earnest

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

A simple method for the simultaneous gas-liquid chromatographic quantitation of fatty acids, sterols and bile acids from human fecal samples is described. The various compounds are directly converted into the n-butyl ester-trimethylsilyl ether derivatives, without prior isolation from the stool. Under these conditions, fecal bile acid derivatives are well resolved from each other and from those of fecal fatty acids and sterols without overlaps. The method was found to be reproducible and recoveries were similar to those obtained after exhaustive solvent extraction of fecal sterols, fatty acids and bile acids. Optimum derivatization conditions that allowed maximum recovery of fecal components with minimal destruction and application of the method for simultaneous bile acid, fatty acid and sterol analysis in human stool are described.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume775
Issue number2
DOIs
StatePublished - Aug 5 2002

Keywords

  • Bile acids
  • Fatty acids
  • Sterols

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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