Single cell level detection of Escherichia coli in microfluidic device

Jin Hee Han, Brian C. Heinze, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Detection of Escherichia coli K-12 in phosphate buffered saline (PBS) was demonstrated in a Y-channel polydimethylsiloxane (PDMS) microfluidic device through optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed for serially diluted E. coli solutions using 0.92-μm highly carboxylated polystyrene particles conjugated with polyclonal anti-E. coli. Pre-treatments such as cell lysis or culturing to enhance the signal were not used. Proximity optical fibers around the view cell of the device were used to quantify the increase in 45° forward light scattering of the immunoagglutinated particles. In order to reduce false positive signals caused by antibodies binding to non-viable E. coli cells or free antigens in solution, target solutions were washed three times, and then the results were compared to non-washing treatments. The detection limit was found to be less than 10 cfu ml-1 (1 cfu per device) without PBS washing (thus detecting non-viable cells and free antigens), or less than 40 cfu ml-1 (4 cfu per device) with PBS washing (thus detecting viable E. coli cells only).

Original languageEnglish (US)
Pages (from-to)1303-1306
Number of pages4
JournalBiosensors and Bioelectronics
Volume23
Issue number8
DOIs
StatePublished - Mar 14 2008

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Escherichia coli
Optical Fibers
Phosphates
Latex
Antigens
Latexes
Washing
Equipment and Supplies
Optical fibers
Forward scattering
Polystyrenes
Polydimethylsiloxane
Antibodies
Light scattering
Limit of Detection
Assays
Light
Monitoring

Keywords

  • Detection limit
  • Escherichia coli K-12
  • Latex agglutination test
  • Latex immunoagglutination assay
  • Microfluidic device
  • Proximity optical fiber
  • Static light scattering

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Single cell level detection of Escherichia coli in microfluidic device. / Han, Jin Hee; Heinze, Brian C.; Yoon, Jeong-Yeol.

In: Biosensors and Bioelectronics, Vol. 23, No. 8, 14.03.2008, p. 1303-1306.

Research output: Contribution to journalArticle

Han, Jin Hee ; Heinze, Brian C. ; Yoon, Jeong-Yeol. / Single cell level detection of Escherichia coli in microfluidic device. In: Biosensors and Bioelectronics. 2008 ; Vol. 23, No. 8. pp. 1303-1306.
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