Single cell targeting using plasmon resonant gold-coated liposomes

Sarah J. Leung, Marek Romanowski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed an experimental system with the potential for the delivery and localized release of an encapsulated agent with high spatial and temporal resolution. We previously introduced liposome-supported plasmon resonant gold nanoshells; in this composite structure, the liposome allows for the encapsulation of substances, such as therapeutic agents, neurotransmitters, or growth factors, and the plasmon resonant structure facilitates the rapid release of encapsulated contents upon laser light illumination. More recently, we demonstrated that these gold-coated liposomes are capable of releasing their contents in a spectrally-controlled manner, where plasmon resonant nanoparticles only release content upon illumination with a wavelength of light matching their plasmon resonance band. We now show that this release mechanism can be used in a biological setting to deliver a peptide derivative of cholecystokinin to HEK293 cells overexpressing the CCK2 receptor. Using directed laser light, we may enable localized release from gold-coated liposomes to enable accurate perturbation of cellular functions in response to released compounds; this system may have possible applications in signaling pathways and drug discovery.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8234
DOIs
StatePublished - 2012
EventPlasmonics in Biology and Medicine IX - San Francisco, CA, United States
Duration: Jan 22 2012Jan 24 2012

Other

OtherPlasmonics in Biology and Medicine IX
CountryUnited States
CitySan Francisco, CA
Period1/22/121/24/12

Fingerprint

Liposomes
Gold
gold
cells
Lighting
illumination
Light
neurotransmitters
Nanoshells
Lasers
composite structures
releasing
Cholecystokinin B Receptor
temporal resolution
peptides
lasers
HEK293 Cells
Cholecystokinin
delivery
drugs

Keywords

  • cellular signaling
  • controlled release
  • liposome
  • nanoparticles
  • plasmon resonance

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Leung, S. J., & Romanowski, M. (2012). Single cell targeting using plasmon resonant gold-coated liposomes. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8234). [82341J] https://doi.org/10.1117/12.909314

Single cell targeting using plasmon resonant gold-coated liposomes. / Leung, Sarah J.; Romanowski, Marek.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8234 2012. 82341J.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Leung, SJ & Romanowski, M 2012, Single cell targeting using plasmon resonant gold-coated liposomes. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8234, 82341J, Plasmonics in Biology and Medicine IX, San Francisco, CA, United States, 1/22/12. https://doi.org/10.1117/12.909314
Leung SJ, Romanowski M. Single cell targeting using plasmon resonant gold-coated liposomes. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8234. 2012. 82341J https://doi.org/10.1117/12.909314
Leung, Sarah J. ; Romanowski, Marek. / Single cell targeting using plasmon resonant gold-coated liposomes. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8234 2012.
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