Single cell transfection dynamics in a microfluidic system

Na Li, Pak Kin Wong, James Lin, Chih Ming Ho

Research output: Contribution to conferencePaper

2 Scopus citations

Abstract

In this study, molecular beacons have been successfully transfected into living cells with high efficiency (80%∼90%) inside a microfluidic system. This will enable a sensitive and specific method to monitor gene expression dynamics in single living cells. Fluorescence conjugated transfection reagent was adopted to investigate the transfection dynamics of molecular beacons. The development of microfluidic systems has facilitated real-time monitoring of individual cells, which revealed the transfection dynamics of molecular beacons at the single cell level.

Original languageEnglish (US)
Pages969-971
Number of pages3
StatePublished - Jan 1 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Keywords

  • Gene expression dynamics
  • Microfluidics
  • Molecular beacon
  • Single cells
  • Transfection

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Li, N., Wong, P. K., Lin, J., & Ho, C. M. (2006). Single cell transfection dynamics in a microfluidic system. 969-971. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.