Single molecule force spectroscopy of the cardiac titin N2B element: Effects of the molecular chaperone αB-crystallin with disease-causing mutations

Yi Zhu, Julius Bogomolovas, Siegfried Labeit, Henk Granzier

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The small heat shock protein αB-crystallin interacts with N2B-Us, a large unique sequence found in the N2B element of cardiac titin. Using single molecule force spectroscopy, we studied the effect of αB-crystallin on the N2B-Us and its flanking Ig-like domains. Ig domains from the proximal tandem Ig segment of titin were also studied. The effect of wild type βB-crystallin on the single molecule force-extension curve was determined as well as that of mutant βB-crystallins harboring the dilated cardiomyopathy missense mutation, R157H, or the desmin-related myopathy mutation, R120G. Results revealed that wild type βB-crystallin decreased the persistence length of the N2B-Us (from ∼0.7 to ∼0.2 nm) but did not alter its contour length. βB-crystallin also increased the unfolding force of the Ig domains that flank the N2B-Us (by 51 ± 3 piconewtons); the rate constant of unfolding at zero force was estimated to be ∼17-fold lower in the presence of βB-crystallin (1.4 × 10-4 s-1 versus 2.4 × 10-3 s-1). We also found that βB-crystallin increased the unfolding force of Ig domains from the proximal tandem Ig segment by 28 ± 6 piconewtons. The effects of αB-crystallin were attenuated by the R157H mutation (but were still significant) and were absent when using the R120G mutant. We conclude that αB-crystallin protects titin from damage by lowering the persistence length of the N2B-Us and reducing the Ig domain unfolding probability. Our finding that this effect is either attenuated (R157H) or lost (R120G) in disease causing βB-crystallin mutations suggests that the interaction between αB-crystallin and titin is important for normal heart function.

Original languageEnglish (US)
Pages (from-to)13914-13923
Number of pages10
JournalJournal of Biological Chemistry
Volume284
Issue number20
DOIs
StatePublished - May 15 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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