Single-pipetting microfluidic assay device for rapid detection of Salmonella from poultry package

Christopher F. Fronczek, David J. You, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A direct, sensitive, near-real-time, handheld optical immunoassay device was developed to detect Salmonella typhimurium in the naturally occurring liquid from fresh poultry packages (hereafter "chicken matrix"), with just single pipetting of sample (i.e., no filtration, culturing and/or isolation, thus reducing the assay time and the error associated with them). Carboxylated, polystyrene microparticles were covalently conjugated with anti-Salmonella, and the immunoagglutination due to the presence of Salmonella was detected by reading the Mie scatter signals from the microfluidic channels using a handheld device. The presence of chicken matrix did not affect the light scatter signal, since the optical parameters (particle size d, wavelength of incident light λ and scatter angle θ) were optimized to minimize the effect of sample matrix (animal tissues and blood proteins, etc.). The sample was loaded into a microfluidic chip that was split into two channels, one pre-loaded with vacuum-dried, antibody-conjugated particles and the other with vacuum-dried, bovine serum albumin-conjugated particles. This eliminated the need for a separate negative control, effectively minimizing chip-to-chip and sample-to-sample variations. Particles and the sample were diffused in-channel through chemical agitation by Tween 80, also vacuum-dried within the microchannels. Sequential mixing of the sample to the reagents under a strict laminar flow condition synergistically improved the reproducibility and linearity of the assay. In addition, dried particles were shown to successfully detect lower Salmonella concentrations for up to 8 weeks. The handheld device contains simplified circuitry eliminating unnecessary adjustment stages, providing a stable signal, thus maximizing sensitivity. Total assay time was 10min, and the detection limit 10CFUmL-1 was observed in all matrices, demonstrating the suitability of this device for field assays.

Original languageEnglish (US)
Pages (from-to)342-349
Number of pages8
JournalBiosensors and Bioelectronics
Volume40
Issue number1
DOIs
StatePublished - Feb 15 2013

Fingerprint

Lab-On-A-Chip Devices
Poultry
Salmonella
Vacuum
Microfluidics
Assays
Equipment and Supplies
Chickens
Light
Optical Devices
Polysorbates
Polystyrenes
Salmonella typhimurium
Bovine Serum Albumin
Immunoassay
Particle Size
Limit of Detection
Blood Proteins
Reading
Optical devices

Keywords

  • Chicken carcass
  • Immunoagglutination
  • Lab on a chip
  • Latex agglutination test
  • Microfluidic device
  • Mie scatter
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Single-pipetting microfluidic assay device for rapid detection of Salmonella from poultry package. / Fronczek, Christopher F.; You, David J.; Yoon, Jeong-Yeol.

In: Biosensors and Bioelectronics, Vol. 40, No. 1, 15.02.2013, p. 342-349.

Research output: Contribution to journalArticle

@article{e041da07be4546bb8c2f8b11cb70a268,
title = "Single-pipetting microfluidic assay device for rapid detection of Salmonella from poultry package",
abstract = "A direct, sensitive, near-real-time, handheld optical immunoassay device was developed to detect Salmonella typhimurium in the naturally occurring liquid from fresh poultry packages (hereafter {"}chicken matrix{"}), with just single pipetting of sample (i.e., no filtration, culturing and/or isolation, thus reducing the assay time and the error associated with them). Carboxylated, polystyrene microparticles were covalently conjugated with anti-Salmonella, and the immunoagglutination due to the presence of Salmonella was detected by reading the Mie scatter signals from the microfluidic channels using a handheld device. The presence of chicken matrix did not affect the light scatter signal, since the optical parameters (particle size d, wavelength of incident light λ and scatter angle θ) were optimized to minimize the effect of sample matrix (animal tissues and blood proteins, etc.). The sample was loaded into a microfluidic chip that was split into two channels, one pre-loaded with vacuum-dried, antibody-conjugated particles and the other with vacuum-dried, bovine serum albumin-conjugated particles. This eliminated the need for a separate negative control, effectively minimizing chip-to-chip and sample-to-sample variations. Particles and the sample were diffused in-channel through chemical agitation by Tween 80, also vacuum-dried within the microchannels. Sequential mixing of the sample to the reagents under a strict laminar flow condition synergistically improved the reproducibility and linearity of the assay. In addition, dried particles were shown to successfully detect lower Salmonella concentrations for up to 8 weeks. The handheld device contains simplified circuitry eliminating unnecessary adjustment stages, providing a stable signal, thus maximizing sensitivity. Total assay time was 10min, and the detection limit 10CFUmL-1 was observed in all matrices, demonstrating the suitability of this device for field assays.",
keywords = "Chicken carcass, Immunoagglutination, Lab on a chip, Latex agglutination test, Microfluidic device, Mie scatter, Salmonella typhimurium",
author = "Fronczek, {Christopher F.} and You, {David J.} and Jeong-Yeol Yoon",
year = "2013",
month = "2",
day = "15",
doi = "10.1016/j.bios.2012.07.076",
language = "English (US)",
volume = "40",
pages = "342--349",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Single-pipetting microfluidic assay device for rapid detection of Salmonella from poultry package

AU - Fronczek, Christopher F.

AU - You, David J.

AU - Yoon, Jeong-Yeol

PY - 2013/2/15

Y1 - 2013/2/15

N2 - A direct, sensitive, near-real-time, handheld optical immunoassay device was developed to detect Salmonella typhimurium in the naturally occurring liquid from fresh poultry packages (hereafter "chicken matrix"), with just single pipetting of sample (i.e., no filtration, culturing and/or isolation, thus reducing the assay time and the error associated with them). Carboxylated, polystyrene microparticles were covalently conjugated with anti-Salmonella, and the immunoagglutination due to the presence of Salmonella was detected by reading the Mie scatter signals from the microfluidic channels using a handheld device. The presence of chicken matrix did not affect the light scatter signal, since the optical parameters (particle size d, wavelength of incident light λ and scatter angle θ) were optimized to minimize the effect of sample matrix (animal tissues and blood proteins, etc.). The sample was loaded into a microfluidic chip that was split into two channels, one pre-loaded with vacuum-dried, antibody-conjugated particles and the other with vacuum-dried, bovine serum albumin-conjugated particles. This eliminated the need for a separate negative control, effectively minimizing chip-to-chip and sample-to-sample variations. Particles and the sample were diffused in-channel through chemical agitation by Tween 80, also vacuum-dried within the microchannels. Sequential mixing of the sample to the reagents under a strict laminar flow condition synergistically improved the reproducibility and linearity of the assay. In addition, dried particles were shown to successfully detect lower Salmonella concentrations for up to 8 weeks. The handheld device contains simplified circuitry eliminating unnecessary adjustment stages, providing a stable signal, thus maximizing sensitivity. Total assay time was 10min, and the detection limit 10CFUmL-1 was observed in all matrices, demonstrating the suitability of this device for field assays.

AB - A direct, sensitive, near-real-time, handheld optical immunoassay device was developed to detect Salmonella typhimurium in the naturally occurring liquid from fresh poultry packages (hereafter "chicken matrix"), with just single pipetting of sample (i.e., no filtration, culturing and/or isolation, thus reducing the assay time and the error associated with them). Carboxylated, polystyrene microparticles were covalently conjugated with anti-Salmonella, and the immunoagglutination due to the presence of Salmonella was detected by reading the Mie scatter signals from the microfluidic channels using a handheld device. The presence of chicken matrix did not affect the light scatter signal, since the optical parameters (particle size d, wavelength of incident light λ and scatter angle θ) were optimized to minimize the effect of sample matrix (animal tissues and blood proteins, etc.). The sample was loaded into a microfluidic chip that was split into two channels, one pre-loaded with vacuum-dried, antibody-conjugated particles and the other with vacuum-dried, bovine serum albumin-conjugated particles. This eliminated the need for a separate negative control, effectively minimizing chip-to-chip and sample-to-sample variations. Particles and the sample were diffused in-channel through chemical agitation by Tween 80, also vacuum-dried within the microchannels. Sequential mixing of the sample to the reagents under a strict laminar flow condition synergistically improved the reproducibility and linearity of the assay. In addition, dried particles were shown to successfully detect lower Salmonella concentrations for up to 8 weeks. The handheld device contains simplified circuitry eliminating unnecessary adjustment stages, providing a stable signal, thus maximizing sensitivity. Total assay time was 10min, and the detection limit 10CFUmL-1 was observed in all matrices, demonstrating the suitability of this device for field assays.

KW - Chicken carcass

KW - Immunoagglutination

KW - Lab on a chip

KW - Latex agglutination test

KW - Microfluidic device

KW - Mie scatter

KW - Salmonella typhimurium

UR - http://www.scopus.com/inward/record.url?scp=84868631352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868631352&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2012.07.076

DO - 10.1016/j.bios.2012.07.076

M3 - Article

VL - 40

SP - 342

EP - 349

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 1

ER -