Site-directed mutagenesis identifies a molecular switch involved in copper sensing by the histidine kinase CinS in Pseudomonas putida KT2440

Davide Quaranta, Megan M. McEvoy, Christopher Rensing

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In the presence of copper, Pseudomonas putida activates transcription of cinAQ via the two-component system CinS-CinR. The CinS-CinR TCS was responsive to 0.5 μM copper and was specifically activated only by copper and silver. Modeling studies of CinS identified a potential copper binding site containing H37 and H147. CinS mutants with H37R and H147R mutations had an almost 10-fold reduced copper-dependent induction of cinAQ compared to the wild type.

Original languageEnglish (US)
Pages (from-to)5304-5311
Number of pages8
JournalJournal of bacteriology
Volume191
Issue number16
DOIs
StatePublished - Aug 2009

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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